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G1163

Sigma-Aldrich

O-Glycosidase from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

Synonym(e):

Endo-α-N-acetylgalactosaminidase, O-Glycanase

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About This Item

CAS-Nummer:
9032-92-2
EC-Nummer:
3.2.1.97 (BRENDA, IUBMB)
EG-Nummer:
232-888-2
MDL-Nummer:
MFCD00131003
UNSPSC-Code:
12352204
NACRES:
NA.32

Rekombinant

expressed in E. coli

Qualitätsniveau

200

Konjugat

(O-linked)

Form

buffered aqueous solution

Mol-Gew.

180 kDa

Konzentration

≥800 units/mL

Versandbedingung

wet ice

Lagertemp.

2-8°C

Biochem./physiol. Wirkung

Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..

Verpackung

Supplied with 5× Reaction Buffer, 250 mM NaH2PO4 pH 5.0.

Einheitendefinition

One unit will hydrolyze 1 μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→ΟC6H4NO2) per min at 37 °C at pH 6.5.

Physikalische Form

Solution in 50 mM sodium phosphate, pH 7.5

Hinweis zur Analyse

Screened for presence of: β-galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases. See Certificate of Analysis for lot specific information.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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H Miyata et al.
Okajimas folia anatomica Japonica, 78(4), 129-140 (2002-01-05)
The distribution or localization of glycoconjugates in rat cerebellar cortex was investigated with 26 different kinds of lectins observed by light and electron microscopy. In paraffin-embedded tissues, PHA-L, PHA-E, DSA, WGA, ConA, LEA, LCA, PSA, and RCA-I, which mainly recognize
K M Hong et al.
Journal of cancer research and clinical oncology, 127(9), 551-558 (2001-09-26)
To identify a new tumor-associated antigen, a monoclonal antibody, SC142, was produced by immunizing mice with a stomach cancer cell line. The tumor specificity of mAb SC142 was studied by immunohistochemical staining, and the biochemical characteristics of this new gastrointestinal
L Robertson et al.
Journal of reproduction and fertility, 120(2), 397-403 (2000-11-04)
This study demonstrates that carbohydrates play an essential role in sperm-egg interactions in birds. Sperm-egg interaction was measured in vitro as the ability of spermatozoa to hydrolyse a small hole in the inner perivitelline layer, the equivalent of the mammalian
Aimee E Stephenson et al.
Molecular microbiology, 43(1), 147-157 (2002-02-19)
Streptococcus parasanguis is a primary colonizer of the tooth surface and plays a pivotal role in the formation of dental plaque. The fimbriae of S. parasanguis are important in mediating adhesion to saliva-coated hydroxylapatite (SHA), an in vitro tooth adhesion
C Liu et al.
Journal of mass spectrometry : JMS, 32(1), 33-42 (1997-01-01)
A high-performance liquid chromatographic/electrospray mass spectrometric (HPLC/MS) technique is described for the characterization of the beta-subunit of the glycopeptide human chorionic gonadotropin (hCG). The beta-subunit of hCG was dissociated from the alpha-subunit using 0.1% trifluoroacetic acid (TFA) and separated by
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Artikel

O-Linked Glycan Strategies

While most N-linked oligosaccharides can be removed using PNGase F, a comparable enzyme for removing intact O-linked sugars has not been identified. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-β(1→3)-GalNAc core remains.

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