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M9004

Sigma-Aldrich

Äpfelsäure-Dehydrogenase aus Rinderherz

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

Synonym(e):

L-Malate:NAD+ oxidoreductase, MDH

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About This Item

EC-Nummer:
1.1.1.37 (BRENDA, IUBMB)
EG-Nummer:
232-622-5
MDL-Nummer:
MFCD00131567
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

bovine heart

Form

ammonium sulfate suspension

Spezifische Aktivität

2000-4000 units/mg protein (modified Warburg-Christian)

Mol-Gew.

35 kDa

Verunreinigungen

≤0.01% Glutamic-Oxalacetic Transaminase
≤0.01% Glutamic-Pyruvic Transaminase

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Anwendung

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

Einheitendefinition

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Physikalische Form

Suspension in 3 M (NH4)2SO4 - 0.01 M KH2PO4 solution, pH 7.3

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Enika Nagababu et al.
Methods in molecular biology (Clifton, N.J.), 610, 165-180 (2009-12-17)
Reactive oxygen species are implicated in many human diseases and aging process. Much of the evidence is based on experimental data indicating increasing rates of lipid peroxidation in disease states and the ameliorating effects of antioxidants. It is becoming increasingly
E M Gregory
The Journal of biological chemistry, 250(14), 5470-5474 (1975-07-25)
Bovine mitochondrial malate dehydrogenase (EC 1.1.1.37) was inactivated by the specific modifications of a single histidine residue upon reaction with iodoacetamide. NADH protected against this loss of activity and reaction with the histidine residue, suggesting that the histidine is at
Eisaku Hokazono et al.
Clinica chimica acta; international journal of clinical chemistry, 412(15-16), 1436-1440 (2011-05-10)
In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis
Flow injection system for on-line monitoring of fumaric acid in biological processes
Rhee, J.
Analytica Chimica Acta, 499, 71-80 (2003)
Isoelectric points and molecular weights of proteins: a new table.
Righetti, P.G., and Tudor, G.
Journal of Chromatography A, 220, 115-194 (1981)
Alle zugehörigen Dokumente anzeigen

Artikel

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protokolle

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

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