M5257

Apfelsäure-Dehydrogenase (Oxalacetat-decarboxylierend) aus Hühnerleber

ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)

• Synonym(e): ‘Malic Enzyme’
• CAS-Nummer: 9028-47-1
• EC-Nummer: 1.1.1.40 (BRENDA, IUBMB)
• EG-Nummer: 232-834-8
• MDL-Nummer: MFCD00131568
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: chicken liver
• Qualitätsniveau: 200
• Form: ammonium sulfate suspension
• Spezifische Aktivität: 10-30 units/mg protein (modified Warburg-Christian)
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.

Anwendung

Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.

Biochem./physiol. Wirkung

Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.

Einheitendefinition

One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO_2, and NADPH per min at pH 7.4 at 25 °C.

Physikalische Form

Suspension in 2.9 M (NH₄)₂SO₄ solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na₄EDTA, pH 6.0

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable