LYSC9001

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, powder

• Synonym(e): API, Lysyl endopeptidase, Protease I
• EC-Nummer: 3.4.21.50 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Achromobacter lyticus
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli (206-473aa)
• Qualität: Proteomics Grade
• Form: lyophilized powder
• Optimaler pH-Wert: 8.5
• pH-Bereich: 9.0-9.5(catalytic)
• UniProt-Hinterlegungsnummer: P15636
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

Spezifität

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

Anwendung

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

Verpackung

1mg/ml in Plastic

Angaben zur Herstellung

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

Rekonstituierung

Reconstitute in sterile water.

Lagerung und Haltbarkeit

Store product at -20°C. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles once the protein has been resolubilized in sterile water.

Sonstige Hinweise

For R&D only.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H315 - H317 - H319 - H334
• P-Sätze: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3