C4249

Cystatin B human

recombinant, expressed in E. coli, solid, ≥95% (SDS-PAGE)

• Synonym(e): CPI-B, CST6, CSTB, EPM1, Liver Thiol Proteinase Inhibitor, PME, STFB, Stefin B
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Cystatin B human, recombinant, expressed in E. coli
• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Beschreibung: contains a N-terminal 7X His-tag
• Assay: ≥95% (SDS-PAGE)
• Form: solid
• Mol-Gew.: apparent mol wt ~13 kDa by SDS-PAGE (reducing)
• NCBI-Hinterlegungsnummer: NP_000091
• Lagertemp.: −20°C
• Angaben zum Gen: human ... CSTB(1476)

Beschreibung

Allgemeine Beschreibung

Cystatin B is a member of the type 1 (stefins) superfamily of cystatins. Cystatin B is encoded by the CSTB gene and functions as an intracellular thiol protease inhibitor regulating the activity of members of the papain family and cathepsins B, H, and L. Cystatin B may also protect cellular components from the effects of proteases that leak from lysosomes. Mutations in the CSTB gene are thought to be the primary defect in patients with Progressive Myoclonus Epilepsy (EPM1).

Anwendung

Suitable for use as a cysteine-protease inhibitor. Is tested for suitability as an inhibitor of the enzymatic activity of papain.

Biochem./physiol. Wirkung

Cystatin B was shown to interact with the protein RACK-1, β-spectrin and neurofilament light chain NF-L forming a unique multiprotein complex specifically in the cerebellum, confirming its function in the brain.

Rekonstituierung

Cystatin B should be reconstituted at 100 μg/mL in steile 25 mM Tris pH 7.5 and 100 mM NaCl. Reconstituted product should be stored in single-use aliquots at -70° C and should be stable for at least 3 months.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable