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A6691

Sigma-Aldrich

Amidase aus Pseudomonas aeruginosa

recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)

Synonym(e):

Acrylamid-Amidohydrolase, Acylase

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About This Item

CAS-Nummer:
9012-56-0
EC-Nummer:
3.5.1.4 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00166319
UNSPSC-Code:
12352204
NACRES:
NA.54

Rekombinant

expressed in E. coli

Qualitätsniveau

200

Form

buffered aqueous glycerol solution

hydroxamate transferase activity

≥200 units/mg protein (biuret)

Konzentration

14 mg/mL

UniProt-Hinterlegungsnummer

Q9HWL8

Lagertemp.

−20°C

Angaben zum Gen

Pseudomonas aeruginosa PAO1 ... PA4163(880181)

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Anwendung

Amidase from Pseudomonas aeruginosa has been used for testing its capability to hydrolyze ochratoxin A.
The importance of these hydrolases in biotechnology is growing rapidly, because their potential applications span through chemical and pharmaceutical industries as well as in bioremediation. Immobilized amidase can be used efficiently for production of acrylic acid from acrylamide, thus converting a toxic ambient contaminant into widely used industrial raw material. Amidases are potential treatments for human immunodeficiency virus and malaria. They may be used to eliminate metal ions in wastewater .

Biochem./physiol. Wirkung

The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer αββα sandwich domain with an additional 81-residue long C-terminal segment .

Einheitendefinition

1 U setzt 1.0 μ;mol Acetamid und Hydroxylamin zu Acetohydroxamat und Ammoniak pro Minute um bei pH 7.2 und 37°C.

Physikalische Form

Lösung in 50% Glycerin, enthält Spuren von 2-Mercaptoethanol und Phosphat-Puffersalz

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

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Lysostaphin aus Staphylococcus staphylolyticus free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein

Sigma-Aldrich

SAE0091

Lysostaphin aus Staphylococcus staphylolyticus

Substrate interaction with recombinant amidase from Pseudomonas aeruginosa during biocatalysis
Pacheco R, Karmali A
Biocatalysis and Biotransformation, 27(5/6), 367-376 (2009)
Jorge Andrade et al.
The Journal of biological chemistry, 282(27), 19598-19605 (2007-04-20)
Microbial amidases belong to the thiol nitrilases family and have potential biotechnological applications in chemical and pharmaceutical industries as well as in bioremediation. The amidase from Pseudomonas aeruginosa isa6 x 38-kDa enzyme that catalyzes the hydrolysis of a small range
Butyramide-utilizing mutants of Pseudomonas aeruginosa 8602 which produce an amidase with altered substrate specificity.
J E Brown et al.
Journal of general microbiology, 57(2), 273-285 (1969-08-01)
Peng Chen et al.
Chemical communications (Cambridge, England), 48(29), 3482-3484 (2012-02-11)
Amidase-catalyzed desymmetrization of meso-N-heterocyclic dicarboxamides under very mild conditions provided a highly efficient and practical method for the preparation of enantiomerically pure carbamoyl-substituted heterocyclic amino acids that were unique and versatile platforms for the construction of both antipodes of aza-sugar
Susanne Berendt et al.
Journal of bacteriology, 194(19), 5218-5227 (2012-07-24)
Filamentous cyanobacteria of the order Nostocales display typical properties of multicellular organisms. In response to nitrogen starvation, some vegetative cells differentiate into heterocysts, where fixation of N(2) takes place. Heterocysts provide a micro-oxic compartment to protect nitrogenase from the oxygen
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