Direkt zum Inhalt
Merck
Alle Fotos(2)

Wichtige Dokumente

Gesamtdokumentation anzeigen

SAE0091

Sigma-Aldrich

Lysostaphin aus Staphylococcus staphylolyticus

free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research

Synonym(e):

Glycyl-glycine Endopeptidase

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(2)

About This Item

CAS-Nummer:
9011-93-2
EC-Nummer:
3.4.24.75 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00131556
UNSPSC-Code:
12352202
NACRES:
NA.54

product name

Lysostaphin aus Staphylococcus staphylolyticus, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein

Biologische Quelle

microbial (Staphylococcus staphylotyticus)

Form

lyophilized powder

Spezifische Aktivität

≥500 units/mg protein

Mol-Gew.

27 kDa

Leistungsmerkmale

DNA free

Versandbedingung

wet ice

Lagertemp.

−20°C

Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich

Anwendung

Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)

Biochem./physiol. Wirkung

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.
Lysostaphin ist eine Zink-Endopeptidase mit einer Molekülmasse von etwa 25 kDa. Da Lysostaphin die Polyglycinvernetzungen in der Peptidoglykanschicht der Zellwand von Staphylococcus-Spezies spaltet, ist es nützlich für die Zelllyse und auch als potenzielles antimikrobielles Therapeutikum.
Optimaler pH-Wert für Aktivität: ~7,5

Einheitendefinition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Physikalische Form

Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Judy J J Ou et al.
Frontiers in cellular and infection microbiology, 6, 187-187 (2017-01-14)
Background:Staphylococcus aureus (S. aureus) small colony variants (SCVs) can survive within the host intracellular milieu and are associated with chronic relapsing infections. However, it is unknown whether host invasion rates and immune responses differ between SCVs and their wild-type counterparts.
Caroline Meyer Olesen et al.
Microorganisms, 9(7) (2021-08-08)
Investigation of changes in the skin microbiome following treatment of atopic dermatitis (AD) with dupilumab may provide valuable insights into the skin microbiome as a therapeutic target. The aim of this study is to assess changes in the AD skin
Ing-Marie Jonsson et al.
PloS one, 5(12), e14209-e14209 (2010-12-15)
Ecs is an ATP-binding cassette (ABC) transporter present in aerobic and facultative anaerobic gram-positive Firmicutes. Inactivation of Bacillus subtilis Ecs causes pleiotropic changes in the bacterial phenotype including inhibition of intramembrane proteolysis. The molecule(s) transported by Ecs is (are) still
Jianjun Miao et al.
Biomaterials, 32(36), 9557-9567 (2011-10-01)
With the emergence of "super bacteria" that are resistant to antibiotics, e.g., methicillin-resistant Staphylococcus aureus, novel antimicrobial therapies are needed to prevent associated hospitalizations and deaths. Bacteriophages and bacteria use cell lytic enzymes to kill host or competing bacteria, respectively
T Baba et al.
The EMBO journal, 15(18), 4789-4797 (1996-09-16)
Microbial organisms secrete antibiotics that cause the selective destruction of specific target cells. Although the mode of action is known for many antibiotics, the mechanisms by which these molecules are directed specifically to their target cells hitherto have not been
Alle zugehörigen Dokumente anzeigen

Artikel

Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease

An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.