SAE0098

Lyticase aus Arthrobacter luteus

free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder

• Synonym(e): (1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
• CAS-Nummer: 37340-57-1
• MDL-Nummer: MFCD00131560
• UNSPSC-Code: 12352202
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: bacterial (Arthrobacter luteus)
• Form: lyophilized powder
• Spezifische Aktivität: ≥2000 units/mg protein
• Leistungsmerkmale: DNA free
• Methode(n): cell based assay: suitable
• Eignung: suitable for cell lysis
• Anwendung(en): diagnostic assay manufacturing
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.

Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Biochem./physiol. Wirkung

Lyticase hydrolysiert Poly-β(1→3)-Glucose wie das Zellwand-Glucan der Hefe.

Einheitendefinition

Eine Einheit produziert ein ΔA₈₀₀ von 0,001 pro min bei pH 7,5 und 25 °C mit einer Hefesuspension als Substrat in 3 ml Reaktionsgemisch.

Sonstige Hinweise

Für mehr Information siehe enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable