A2289
Albumin, Tetramethylrhodamine isothiocyanate bovine
lyophilized powder
Synonym(e):
TRITC-albumin
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Alle Fotos(2)
About This Item
Empfohlene Produkte
Biologische Quelle
bovine
Qualitätsniveau
Assay
≥90% (Lowry)
Form
lyophilized powder
Konzentration
≥90% protein (Lowry)
Kennzeichnungsgrad
≥0.5 mol TRITC per mol albumin
Methode(n)
cell culture | mammalian: suitable
Verunreinigungen
salt, free
UniProt-Hinterlegungsnummer
Lagertemp.
2-8°C
Angaben zum Gen
bovine ... ALB(280717)
Allgemeine Beschreibung
Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).
Anwendung
Albumin, Tetramethylrhodamine isothiocyanate has been used:
- in photoinduced release assay of polyethylene beads
- for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
- in fluorescent correlation spectroscopy
Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.
Angaben zur Herstellung
Prepared from crystallized and lyophilized albumin (A4378).
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
13 - Non Combustible Solids
WGK
WGK 3
Persönliche Schutzausrüstung
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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