A1287

5-Azacytidin

Hybri-Max^™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

• Synonym(e): 4-Amino-1-(β-D-ribofuranosyl)-1,3,5-triazin-2(1H)-on, Ladakamycin
• Empirische Formel (Hill-System): C₈H₁₂N₄O₅
• CAS-Nummer: 320-67-2
• Molekulargewicht: 244.20
• Beilstein: 620461
• EG-Nummer: 206-280-2
• MDL-Nummer: MFCD00006539
• UNSPSC-Code: 12352207
• PubChem Substanz-ID: 24890525
• NACRES: NA.75

Eigenschaften

• Biologische Quelle: synthetic (organic)
• Qualitätsniveau: 200
• Qualität: Hybri-Max^™
• Sterilität: γ-irradiated
• Produktlinie: BioXtra
• Form: lyophilized powder
• Methode(n): cell culture | hybridoma: suitable
• Verunreinigungen: endotoxin, tested
• mp (Schmelzpunkt): 226-232 °C (dec.) (lit.)
• Löslichkeit: acetic acid: water (1:1): 5 mg/mL, clear, colorless
• Wirkungsspektrum von Antibiotika: viruses
• Wirkungsweise: DNA synthesis | interferes
• Lagertemp.: −20°C
• SMILES String: NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
• InChI: 1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1
• InChIKey: NMUSYJAQQFHJEW-KVTDHHQDSA-N
• Angaben zum Gen: human ... DNMT1(1786) , DNMT3A(1788)

Beschreibung

Allgemeine Beschreibung

Chemical structure: nucleoside

Anwendung

5-Azacytidine may be used to induce in vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. It may also be used to study the effects of 5-Azacytidine on the immune regulatory myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs).

Used to study the effects of the DNA incorporated molecule on DNA methylation processes.

Biochem./physiol. Wirkung

Ein wirksamer Wachstumshemmer und stark zytotoxischer Wirkstoff; hemmt die DNA-Methyltransferase, ein wichtiger Regulationsmechanismus der Genexpression und Genaktivierung und -stilllegung.

5-Azacytidine is a deoxycytidine analog and a demethylating agent. It acts as a potential antineoplastic agent for acute myelogenous leukemia. 5-Azacytidine activates repressed genes by inhibiting DNA methylation. 5-Azacytidine also affects protein synthesis by altering the RNA function and stability.

Rekonstituierung

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500ml medium. Final working concentration: 10 μM 5-azacytidine.

Rechtliche Hinweise

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Piktogramme: GHS08,GHS07,GHS09
• Signalwort: Danger
• H-Sätze: H302,H341,H350,H360FD,H372,H410
• P-Sätze: P202 - P260 - P264 - P273 - P301 + P312 - P308 + P313
• Gefahreneinstufungen: Acute Tox. 4 Oral - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1B - STOT RE 1
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges