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SH1131

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, Lentiviral Particles

Nuclear Hormone Receptors

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About This Item

Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51

Niveau de qualité

200

Gamme de produits

MISSION®

Température de stockage

−70°C

Informations sur le gène

human ... AR(367) , ESR1(2099) , ESR2(2100) , ESRRA(2101) , ESRRB(2103) , ESRRG(2104) , HNF4A(3172) , HNF4G(3174) , NR0B1(190) , NR0B2(8431) , NR1D1(9572) , NR1D2(9975) , NR1H2(7376) , NR1H3(10062) , NR1H4(9971) , NR1I2(8856) , NR1I3(9970) , NR2C1(7181) , NR2C2(7182) , NR2E1(7101) , NR2E3(10002) , NR2F2(7026) , NR2F6(2063) , NR3C2(4306) , NR4A1(3164) , NR4A2(4929) , NR4A3(8013) , NR5A1(2516) , NR5A2(2494) , NR6A1(2649) , PGR(5241) , PPARA(5465) , PPARD(5467) , PPARG(5468) , RARA(5914) , RARB(5915) , RARG(5916) , RORA(6095) , RORB(6096) , RORC(6097) , RXRA(6256) , RXRB(6257) , RXRG(6258) , THRA(7067) , THRB(7068) , VDR(7421)

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Description générale

Lentiviral particles (representational, plate titer) are provided in 96-well plates that are barcoded for simple identification. Aliquots of 4 x 50μl are provided to prevent the need for excessive freeze/thaw, and maximize functional viral particles. Each gene family set is representationally titered (10% of clones). Fully titered sets are also available, for more information inquire at RNAi@sial.com. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Autres remarques

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 218, Number of Clones: 1448
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Informations légales

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Nuclear hormone receptor assays for drug discovery
Rosen, J., et al.
Drug Des. Discovery, 6, 224-230 (2003)
The nuclear hormone receptor gene superfamily
Ribeiro, R. C., et al.
Annals of Medicine, 46, 443-453 (1995)
Coactivators in assay design for nuclear hormone receptor drug discovery
Chen, T., et al.
Drug Delivery Technology, 1, 835-842 (2003)
Miltiadis Kininis et al.
Nuclear receptor signaling, 6, e005-e005 (2008-02-28)
Recent genomic analyses of transcription factor binding, histone modification, and gene expression have provided a global view of transcriptional regulation by nuclear receptors (NRs) that complements an existing large body of literature on gene-specific studies. The picture emerging from these
J C Burns et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(17), 8033-8037 (1993-09-01)
The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene transfer in eukaryotic cells. To overcome these limitations, we have produced murine leukemia virus-derived vectors in which the retroviral envelope glycoprotein has been completely
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