Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Key Documents

View All Documentation

11218590910

Roche

DNA Molecular Weight Marker II, DIG-labeled

greener alternative

solution, pkg of 500 μL (10 μg/ml)

Synonyme(s) :

DNA marker

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
41105335

Forme

solution

Niveau de qualité

100

Conditionnement

pkg of 500 μL (10 μg/ml)

Fabricant/nom de marque

Roche

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Concentration

10 μg/mL

Couleur

colorless

Solubilité

water: miscible

Autre catégorie plus écologique

, Aligned

Température de stockage

−20°C

Catégories apparentées

Description générale

DNA Molecular Weight Marker II, DIG-labeled is a solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. This fragment mixture is prepared by cleavage of λDNA with Hind III. The size of the fragment ranges from 0.12 to 23.1 kbp. A photodigoxigenin has been introduced at approx. every 200th to 300th base in the DNA fragments.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DNA Molecular Weight Marker II, DIG-labeled has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection.

Caractéristiques et avantages

Contents
Ready-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.

Séquence

The mixture contains 8 fragments with the following base pair lengths: 125, 564, 2027, 2322, 4361, 6557, 9416, and 23130 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 2

1 of 2

DIG Easy Hyb™ Granules powder, suitable for hybridization

Roche

11796895001

DIG Easy Hyb™ Granules

DIG Easy Hyb™ suitable for hybridization, solution

Roche

11603558001

DIG Easy Hyb™

Yingkun Sun et al.
BMC plant biology, 14, 288-288 (2014-10-26)
Double flower domestication is of great value in ornamental plants and presents an excellent system to study the mechanism of morphological alterations by human selection. The classic ABC model provides a genetic framework underlying the control of floral organ identity
The APETALA1 and FRUITFUL homologs in Camellia japonica and their roles in double flower domestication
Yingkun Sun
Molecular breeding : new strategies in plant improvement, 33(4), 821-834 (2014)
Haipeng Xue et al.
Methods in molecular biology (Clifton, N.J.), 1307, 173-190 (2014-03-13)
Genetic modification is an indispensable tool to study gene function in normal development and disease. The recent breakthrough of creating human induced pluripotent stem cells (iPSCs) by defined factors (Takahashi et al., Cell 131:861-872, 2007) provides a renewable source of
Yongquan Luo et al.
Current protocols in stem cell biology, 29, 5A-5A (2014-05-20)
Generation of a fluorescent GFP reporter line in human induced pluripotent stem cells (hiPSCs) provides enormous potentials in both basic stem cell research and regenerative medicine. A protocol for efficiently generating such an engineered reporter line by gene targeting is

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique