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Key Documents

11218603910

Roche

DNA Molecular Weight Marker III, DIG-labeled

greener alternative

10 μg/mL, pkg of 500 μL (10 μg/ml), solution

Synonyme(s) :

DNA marker

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About This Item

Code UNSPSC :
41105335

Forme

solution

Niveau de qualité

Conditionnement

pkg of 500 μL (10 μg/ml)

Fabricant/nom de marque

Roche

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Concentration

10 μg/mL

Couleur

colorless

Solubilité

water: miscible

Autre catégorie plus écologique

Température de stockage

−20°C

Description générale

DNA Molecular Weight Marker III, DIG-labeled is a fragment mixture prepared by cleavage of λDNA with EcoR I and Hind III. The size ranges from 0.12 to 21.2kbp. A photodigoxigenin has been introduced at approx. every 200th to 300th base in the DNA fragments.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DNA Molecular Weight Marker III, DIG-labeled, has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection.

Caractéristiques et avantages

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Séquence

The mixture contains 13 fragments wit the following base pair lengths: 125, 564, 831, 947, 1375, 1584, 1904, 2027, 3530, 4268, 4973, 5148, and 21226 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Autres remarques

For life science research only. Not for use in diagnostic procedures.
Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker the smallest fragments will only be visible on overloaded gels.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available


Certificats d'analyse (COA)

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Diane M Ramos et al.
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Precise temporal and spatial regulation of transgene expression is a critical tool to investigate gene function in developing organisms. The most commonly used technique to achieve tight control of transgene expression, however, requires the use of specific DNA enhancers that
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PLoS currents, 6, doi:10-doi:10 (2014-03-13)
Many human diseases are associated with the abnormal expansion of unstable trinucleotide repeat sequences. The mechanisms of trinucleotide repeat size mutation have not been fully dissected, and their understanding must be grounded on the detailed analysis of repeat size distributions

Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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