233155

Cleland′s Reagent

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.

• Synonyme(s) : Cleland’s Reagent, DTT, Dithiothreitol, Dithiothreitol, DTT
• Formule empirique (notation de Hill): C₄H₁₀O₂S₂
• Numéro CAS: 3483-12-3
• Poids moléculaire : 154.25
• Numéro MDL: MFCD00004877
• Code UNSPSC : 12161504
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Pureté: ≥97% (titration)
• Forme: powder
• Pertinence de la réaction: reagent type: reductant
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Couleur: white to off-white
• Solubilité: water: 30 mg/mL; ethanol: soluble
• Traces de cations: heavy metals: ≤1 ppm; heavy metals: <1 ppm
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• Clé InChI: VHJLVAABSRFDPM-IMJSIDKUSA-N

Description

Description générale

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.

DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.

Oxidized dithiothreitol: ≤0.5%.

Application

• Cyclic nucleotide phosphodiesterase in Dictyostelium discoideum: interconversion of two enzyme forms.: Discusses the role of Cleland′s Reagent in differentiating forms of cyclic nucleotide phosphodiesterase, showcasing its utility in enzymatic studies and its broader implications in biochemistry research (Chassy BM, 1972).

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Gailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302,H315,H318
• Conseils de prudence: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Classification des risques: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable