233156

Cleland′s Reagent, Molecular Biology Grade

A protective agent for SH groups.

• Synonyme(s) : Cleland’s Reagent, Molecular Biology Grade, 1,4-Dithiothreitol, DTT
• Formule empirique (notation de Hill): C₄H₁₀O₂S₂
• Numéro CAS: 3483-12-3
• Poids moléculaire : 154.25
• Numéro MDL: MFCD00004877
• Code UNSPSC : 12161504
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 100
• Pureté: ≥99% (titration)
• Forme: powder; solid
• Pertinence de la réaction: reagent type: reductant
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Couleur: white
• Solubilité: water: 15 mg/mL
• Traces de cations: heavy metals: ≤0.001%
• Absorption: ≤0.100 at 280 nm at 100 mM (1 cm); ≤0.500 at 260 nm at 100 mM (1 cm)
• Activité étrangère: DNases, proteases, RNases, none detected
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• Clé InChI: VHJLVAABSRFDPM-IMJSIDKUSA-N

Description

Description générale

A protective agent for SH groups.

Water-soluble protective reagent for sulfhydryl groups [absorbance (100 mM): ≤0.5 at 260 nm; ≤0.1 at 280 nm]. It is capable of maintaining monothiols completely in the reduced state and of reducing disulfides quantitatively. Since it readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation.

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302,H315,H318
• Conseils de prudence: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Classification des risques: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable