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HPLC Analysis of Proteins on BIOshell A160 Peptide C18

HPLC Analysis of Proteins on BIOshell A160 Peptide C18 application for HPLC

Materiais

Padrão

Nº do produto
Descrição
Preços

T9128

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder

A2327

Adrenomedullin 52 human

≥90% (HPLC), powder

R6513

Ribonuclease A

≥70 Kunitz units/mg protein, lyophilized

62971

Lisozima

powder (crystalline), 70000-140000 U/mg

S7571

Superóxido dismutase

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

41235

Ovalbumin-Atto 488 conjugate

suitable for fluorescence

C6749

Cytochrome c equine

recombinant, expressed in E. coli

C2624

Anidrase carbônica

≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

coluna analítica

Nº do produto
Descrição
Preços

53307-U

Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile

gradient

held at 0% B for 0.5 min; 0 to 100% B in 7 min

flow rate

0.3 mL/min

pressure

4743 psi (327 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

1 μL

sample

0.2 - 0.6 g/L in mobile phase A

Descrição

Informações legais

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Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS