HPLC Analysis of Caffeine Metabolites and Analogs on Discovery^® C18

column

Discovery C18, 15 cm x 4.6 mm I.D., 5 μm particles (504955)

mobile phase

[A] 1% acetic acid in water; [B] methanol; (92:8, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 254 nm