823425

TOYOPEARL^® AF-rProtein A HC-650F Bulk Media

phase rProtein A, bottle of 10 mL, 45 μm particle size

• Sinônimo(s): TOYOPEARL^® AF-rProtein A HC-650F Bulk Media
• Código UNSPSC: 41106500

Propriedades

• descrição: Adsorption capacity (> 80 g/L IgG (static); >70 g/L IgG (dynamic at 5 min. residence time)); Clean in Place (CIP) (0.1 M NaOH); Phase filter (Protein A)
• linha de produto: TOYOPEARL^®
• forma: slurry
• embalagem: bottle of 10 mL
• fabricante/nome comercial: Tosoh 23425
• Parâmetros: 3 bar max. pressure
• técnica(s): LPLC: suitable
• matriz: hydroxylated methacrylic polymer particle platform
• Grupo ativo da matriz: rProtein A phase
• tamanho de partícula: 45 μm
• tamanho de poro: 1000 Å
• pH: 2-13
• capacidade: >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time); >80 g/L adsorption capacity (IgG)(static)
• técnica de separação: affinity
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

TOYOPEARL AF-rProtein A HC-650F is a high capacity Protein A resin for monoclonal antibody purification. An enhanced rProtein A ligand is bound to the TOYOPEARL HW-65F base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycles with 0.1 mol/L NaOH.
TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.

Especificações

Clean in place with 0.1 M NaOH.

forma física

72% (v/v) slurry in 20% (v/v) ethanol

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Informações legais

Toyopearl is a registered trademark of Tosoh Corporation

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P210 - P233 - P240 - P241 - P242 - P243
• Classificações de perigo: Flam. Liq. 3
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): 104.0 °F - closed cup
• Ponto de fulgor (°C): 40 °C - closed cup