ZRB2589

Anti-TCF21 Antibody, clone 2H11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinônimo(s): Capsulin, Class A basic helix-loop-helix protein 23, Epicardin, Podocyte-expressed 1, Pod-1, Transcription factor 21, bHLHa23
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 2H11, recombinant monoclonal
• descrição: recombinant, expressed in HEK 293 cells
• linha de produto: ZooMAb^® learn more
• forma: lyophilized
• peso molecular: calculated mol wt 20 kDa; observed mol wt ~20 kDa
• purificado por: using Protein A
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): mouse, rat
• embalagem: antibody small pack of 25
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnica(s): affinity binding assay: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG
• sequência de epítopo: N-terminal half
• nº de adesão de ID de proteína: NP_003197
• nº de adesão UniProt: O43680
• categoria alternativa mais ecológica: Aligned
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: human ... TCF21(6943)

Descrição

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone 2H11 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Transcription factor 21 (TCF21). It targets an epitope within 17 amino acids from the N-terminal half.

Imunogênio

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human Transcription factor 21 (TCF21).

Aplicação

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TCF21 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TCF21 in A549 cell lysate.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected TCF21 in one million A549 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TCF21 in Human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound TCF21 peptide with a KD of 3.1 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Transcription factor 21 (UniProt: O43680; also known as TCF-21, Capsulin, Class A basic helix-loop-helix protein 23, bHLHa23, Epicardin, Podocyte-expressed 1, Pod-1) is encoded by the TCF21 (also known as BHLHA23, POD1) gene (Gene ID: 6943) in human. TCF21 is a transcription factor of the basic helix-loop-helix (bHLH) family that is involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. It bHLH domain is localized in amino acids 79-131. Its efficient DNA binding requires dimerization with another bHLH protein. It forms a heterodimer with TCF3 and binds the E box (5′-CANNTG-3′). TCF21 is specific for mesoderm and is expressed in the embryo mesenchymal derived tissues, such as the epicardium. It is reported to play a vital role in regulating cell differentiation and cell fate specificity through epithelial-mesenchymal transformation during cardiac development. TCF21 is shown to disrupt the central SRF/MYOCD interaction via multiple mechanisms, suggesting that it may also serve a master regulatory function in promoting smooth muscle cells de-differentiation. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Xie, Y., and Martin, KA (2020). Cir. Res. 126(4); 530-532; Hu, H., et al. (2020). Front. Cell Dev. Biol. 8; 457).

forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstituição

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable