ZRB1780

Anti-mTOR Antibody, clone 4O5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Código UNSPSC: 12352203

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 4O5, monoclonal; recombinant monoclonal
• linha de produto: ZooMAb^® learn more
• forma: lyophilized
• peso molecular: calculated mol wt ~288.89 kDa; observed mol wt ~290 kDa
• reatividade de espécies: mouse, rat, human
• embalagem: antibody small pack of 25 μL
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• técnica(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG
• nº de adesão UniProt: P42345
• categoria alternativa mais ecológica: , Aligned
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone 4O5 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects mTOR. It targets an epitope within 24 amino acids from the C-terminal half.

Imunogênio

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human mTOR.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected mTOR in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected mTOR in A549, L6, and C2C12 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected mTOR in human testis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected mTOR in A549 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-mTOR, clone 4O5 ZooMAb^®, Cat. No. ZRB1780, is a rabbit recombinant monoclonal antibody that specifically targets mTOR and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Descrição-alvo

Serine/threonine-protein kinase mTOR (UniProt: P42345; also known as EC:2.7.11.1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1) is encoded by the MTOR (also known as FRAP, FRAP1, FRAP2, RAFT1, RAPT1) gene (Gene ID: 2475) in human. mTOR is an evolutionarily conserved serine/threonine kinase that acts as a nutrient sensor and regulates growth factor signaling and cellular metabolism. Its catalytic kinase domain located in the C-terminal region exhibits a strong homology to the catalytic domain of PI 3-kinase and PI 4-kinase. It activates translation initiation in response to both hormonal and nutrient signals by phosphorylating at least two translational modulators, p70S6 kinase 1 (S6K1) and eIF-4E-binding protein 1. Cellular mTOR is composed of two large protein complexes, known as mTORC1 and mTORC2. The mTORC1 complex is sensitive to rapamycin and consists of mTOR, Raptor, and mLST8 and two negative regulators, PRAS40 and DEPTOR. The Raptor unit serves as a scaffolding protein to bridge the interaction between mTOR and its substrates. The mTORC2 complex that is insensitive to rapamycin, contains mTOR, Rictor, Sin1, and mLST8 and the newly identified components Protor, Hsp70 and DEPTOR. This complex plays a role in actin cytoskeleton organization and phosphorylates Akt on Ser473 to fully activate it. Deregulation of multiple elements of the mTOR pathway has been reported in many types of cancers. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstituição

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable