Pular para o conteúdo
Merck
Todas as fotos(11)

Key Documents

ZMS1043

Sigma-Aldrich

Anti-Bestrophin-1 Antibody, clone E6-6 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

E6-6, recombinant monoclonal
monoclonal

linha de produto

ZooMAb® learn more

forma

lyophilized

peso molecular

calculated mol wt ~67.68 kDa
observed mol wt ~80 kDa

reatividade de espécies

mouse, human, rabbit

reatividade da espécie (prevista por homologia)

monkey, porcine, bovine

embalagem

antibody small pack of 25 μL

características do produto alternativo mais ecológico

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validação aprimorada

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

técnica(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotipo

IgG1

nº de adesão UniProt

categoria alternativa mais ecológica

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone E6-6 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Bestrophin-1. It targets an epitope within 18 amino acids from the C-terminal region.

Imunogênio

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Bestrophin-1.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in Human retina tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Bestrophin-1 in Human retina tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Bestrophin-1 in Mouse and Rabbit Retina tissue lysates.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Bestrophin-1 in human retina tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Bestrophin-1 in human retina tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Bestrophin-1, clone E6-6 ZooMAb®, Cat. No. ZMS1043, is a recombinant Mouse monoclonal antibody that specifically targets Bestrophin-1 and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Descrição-alvo

Bestrophin-1 (UniProt: O76090; also known as TU15B, Vitelliform macular dystrophy protein 2) is encoded by the BEST1 (also known as VMD2) gene (Gene ID: 7439) in human. Bestrophin-1 is a multi-pass, homopentameric membrane protein that contains three cytoplasmic domains, three extracellular domains, and four transmembrane domains. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. However, its expression has also been reported in cortical and hippocampal astrocytes and in cerebellar Bergmann glia and lamellar astrocytes. It serves as a calcium-activated anion channel and its calcium-binding clasp consists of the acidic cluster of amino acids Glu300, Asp301, Asp302, Asp303, and Asp4304). Under physiological conditions, Bestrophin-1 is also involved in glutamate and GABA release from astrocytes to modulate neuronal excitability, synaptic transmission, and synaptic plasticity. Mutations in BEST1 gene have been linked to various macular dystrophies and retinitis pigmentosa that is characterized by retinal pigment deposits and primary loss of photoreceptor cells followed by secondary loss of cone photoreceptors. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oh, S-J., et al. (2017). Exp. Neurobiol. 26(3); 113-121).

forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstituição

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Não está encontrando o produto certo?  

Experimente o nosso Ferramenta de seleção de produtos.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica