T8573

Monoclonal Anti-Topoisomerase I antibody produced in mouse

clone Mab1, purified immunoglobulin, buffered aqueous solution

• Número MDL: MFCD03097334
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: Mab1, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~100 kDa
• reatividade de espécies: human
• técnica(s): microarray: suitable; western blot: 1-2 μg/mL using A231 human kidney cells
• Isotipo: IgG1
• nº de adesão UniProt: P11387
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TOP1(7150)

Descrição

Descrição geral

Monoclonal Anti-Topoisomerase I (mouse IgG1 isotype) is derived from the Mab1 hybridoma produced by the fusion of SP2/0 mouse myeloma cells and splenocytes from BALB/c mice immunized with human purified topoisomerase I. The protein family of DNA topoisomerases is divided into two types (type I and II). The type I enzymes include eukaryotic and bacterial topoisomerase I and III. Human DNA Topoisomerase I belong to the subtype IB enzymes. The protein has four distinct domains. There are four nuclear localization signals in the N-terminal domain. A linker domain of 77 amino acids follows the core domain. The fourth domain, which is 53 amino acids long, contains the active site Tyr⁷²³. Topoisomerase I (TOP1) gene is located on human chromosome 20q12.

Imunogênio

purified human topoisomerase I

Aplicação

Monoclonal Anti-Topoisomerase I antibody produced in mouse has been used in western blot analysis.

Ações bioquímicas/fisiológicas

DNA topoisomerases are enzymes that control the amount of supercoiling in DNA. Without topoisomerases, DNA cannot replicate normally. Type I enzymes of DNA topoisomerases introduce transient single strand breaks in DNA. Recently it was reported that DNA topoisomerase I is involved in modulation of RNA and is present in retroviral particles, like human immunodeficiency virus (HIV1). Furthermore, the enzyme enhances HIV-1 cDNA production in reverse transcription assays in vitro. Topoisomerase I (TOP1) serves as swivel at the time of replication, transcription and recombination, which helps in releasing over winding of duplex DNA.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable