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T7024

Sigma-Aldrich

N,N,N′,N′-tetrametiletilenodiamina

BioReagent, for molecular biology, ≥99% (GC)

Sinônimo(s):

1,2-Bis(dimetilamino)etano, TEMED, TMEDA

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About This Item

Fórmula linear:
(CH3)2NCH2CH2N(CH3)2
Número CAS:
110-18-9
Peso molecular:
116.20
Beilstein:
1732991
Número CE:
203-744-6
Número MDL:
MFCD00008335
Código UNSPSC:
41105319
ID de substância PubChem:
24900242
NACRES:
NA.31

grau

for molecular biology

Nível de qualidade

200

linha de produto

BioReagent

Ensaio

≥99% (GC)

Lim. expl.

9.08 %

índice de refração

n20/D 1.4179 (lit.)

pb

120-122 °C (lit.)

pf

−55 °C (lit.)

densidade

0.775 g/mL at 20 °C (lit.)

atividade externa

DNase, RNase, protease, none detected

cadeia de caracteres SMILES

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

chave InChI

KWYHDKDOAIKMQN-UHFFFAOYSA-N

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Aplicação

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Quantidade

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Palavra indicadora

Danger

Frases de perigo

H225,H302,H314,H331

Classificações de perigo

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

61.7 °F - closed cup

Ponto de fulgor (°C)

16.5 °C - closed cup

Equipamento de proteção individual

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Persulfato de amônio ACS reagent, ≥98.0%

Sigma-Aldrich

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Persulfato de amônio

Acrylamide solution 40%, suitable for electrophoresis, sterile-filtered

Sigma-Aldrich

A4058

Acrylamide solution

Acrilamida suitable for electrophoresis, ≥99%

Sigma-Aldrich

A8887

Acrilamida

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Sigma-Aldrich

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H Rosing et al.
Journal of chromatography. B, Biomedical applications, 668(1), 107-115 (1995-06-09)
A sensitive high-performance liquid chromatographic (HPLC) assay has been developed and validated for the quantitation of the novel anticancer agent topotecan and topotecan as the total of its lactone and carboxylate forms in human plasma. Linear response in analyte standard
G Patras et al.
Electrophoresis, 20(10), 2039-2045 (1999-08-18)
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox
Deepa Mohan et al.
Journal of chromatography. A, 979(1-2), 271-276 (2002-12-25)
The non-availability of commercial carrier ampholytes in the pH range greater than 11 has contributed to difficulties in focusing and resolving highly basic proteins/peptides using capillary isoelectric focusing (cIEF). Two different approaches, involving the use of N,N,N',N'-tetramethylethylenediamine (TEMED) and ampholyte
R Barberi et al.
Journal of chromatography. B, Biomedical applications, 683(1), 3-13 (1996-08-09)
Atomic force microscopy (AFM) has been used to probe the surface of a capillary after coating with "soft" polymers, notably polyacrylamides. The aim was the investigation of the efficiency of coverage of the silica surface, so as to reduce or
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
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