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Sigma-Aldrich

TransIT-CRISPR®

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About This Item

Código UNSPSC:
12352200

aplicação(ões)

CRISPR

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Descrição geral

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Características e benefícios

  • Highly efficient, non-liposomal polymeric transfection/delivery method for use with MISSION CRISPR/Cas9 DNA, RNA, Cas9 RNP Complexes and siRNA.
  • TransIT-CRISPR is a simple, fast, effective, and versatile. One transfection reagent to deliver CRISPR-Cas9 components in a variety of formats.
  • Effective in most cell lines including difficult-to-transfect cells.

Componentes

0.4ml and 1ml DNA and siRNA non-liposomal, polymeric transfection reagent

Ligação

To order MISSION® CRISPR reagents, please click here

For more information and transfection protocols, please visit SigmaAldrich.com/TransITCRISPR

Outras notas

  • TransIT-CRISPR has been tested in variety of cell types including U20S and HEK 293, A549 and HeLa among others.
  • It is recommended to transfect MISSION CRISPR DNA and RNA with the TransIT-CRISPR transfection reagent.
  • Sigma-Aldrich is the exclusive distributor of TransIT-CRISPR from Mirus Bio LLC.

Informações legais

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TransIT-CRISPR is a registered trademark of Mirus Bio LLC

Pictogramas

FlameExclamation mark
GHS02,GHS07

Palavra indicadora

Danger

Frases de perigo

H225 - H319

Classificações de perigo

Eye Irrit. 2 - Flam. Liq. 2

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

57.2 °F - closed cup

Ponto de fulgor (°C)

14.0 °C - closed cup

Certificados de análise (COA)

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Protocolos

SygRNA® Synthetic CRISPR Guide RNAs

Sigma-Aldrich® sgRNA, crRNA and tracrRNA come HPLC purified as standard with our performance guarantee—even for custom designs.

Ribonucleoprotein (RNP) Protocols using SygRNA ® gRNAs and Cas9 proteins

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Conteúdo relacionado

Gene Expression & Silencing

Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).

Visualize your CRISPR Experiment with MISSION

Watch Cas9-GFP proteins work inside your cells and deliver the powerful nuclease editing you have come to trust and expect. MISSION™ SpCas9-GFP and eSpCas9-GFP proteins are multi-functional enzymes with high on-target cleavage efficiency fused to Enhanced Green Fluorescent Protein (EGFP) for real-time observation of Cas9 delivery and clearance from cells.

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