T0319

Trizma^® Pre-set crystals

BioPerformance Certified, pH 7.4, average M_w 151.6

• Sinônimo(s): Tris crystals
• Código UNSPSC: 12161700
• NACRES: NA.25

Propriedades

• grau: BioPerformance Certified
• Nível de qualidade: 200
• Formulário: crystalline powder
• peso molecular: average M_w 151.6
• técnica(s): cell culture | mammalian: suitable
• Impurezas: DNase, RNase, Protease, NICKase, none detected; endotoxin, tested; total aerobic microbial count, tested; ≤0.05 A₂₉₀; ≤0.5% water (Karl Fischer); ≤5 ppm Heavy metals (as Pb)
• pH: 7.12 (37 °C, 0.1 M)(lit.); 7.40 (25 °C, 0.1 M)(lit.); 7.4; 7.97 (5 °C, 0.1 M)(lit.)
• faixa de pH útil: 7.0-9.0
• solubilidade: deionized water: soluble at 25 °C, clear, colorless (pH 7.4, 1 pouch dissolved in 1 L to yield a 0.1 M Trizma solution)
• aplicação(ões): diagnostic assay manufacturing

Descrição

Aplicação

Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Embalagem

In convenient foil pouches

Outras notas

This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Nota de preparo

Trizma solutions can be autoclaved.

Informações legais

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves