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SRP5272

Sigma-Aldrich

SIRT5, active, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinônimo(s):

SIR2L5

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

recombinante

expressed in baculovirus infected Sf9 cells

Ensaio

≥70% (SDS-PAGE)

Formulário

buffered aqueous glycerol solution

atividade específica

17.0-26.5 RLU/min·ng

peso molecular

~39 kDa

nº de adesão NCBI

NM_012241

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... SIRT5(23408)

Descrição geral

SIRT5 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT5 being a member of class III. SIRT5 consists of eight exons and is found in two isoforms which encode a 310 aa and a 299 aa protein, respectively. Human SIRT5 is most predominantly expressed in heart muscle cells and in lymphoblasts. Fluorescence in situ hybridization analysis localized the human SIRT5 gene to chromosome 6p23. SIRT5 can deacetylate cytochrome c, a protein of the mitochondrial intermembrane space with a central function in oxidative metabolism as well as apoptosis initiation.

Ações bioquímicas/fisiológicas

Sirtuins are NAD+-dependent deacetylases. Sirtuin 5 (SIRT5) catalyzes the degradation of negatively charged acylated substrates. It also possesses desuccinylase, demalonylase and deglutarylase activities. The protein is overexpressed in non-small cell lung cancer (NSCLC) and may have a role in cancer growth.

forma física

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Nota de preparo

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Frases de perigo

H315,H319,H360D

Classificações de perigo

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
E297-1

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Visite a Biblioteca de Documentos

Role of the Substrate Specificity-Defining Residues of Human SIRT5 in Modulating the Structural Stability and Inhibitory Features of the Enzyme.
Yu J
PLoS ONE, 11(3), e0152467-e0152467 (2016)
Assignment of the NAD-dependent deacetylase sirtuin 5 gene (SIRT5) to human chromosome band 6p23 by in situ hybridization.
Mahlknecht U
Cytogenetic and genome research, 112(3-4), 208-212 (2006)
Chemical probing of the human sirtuin 5 active site reveals its substrate acyl specificity and peptide-based inhibitors.
Roessler C
Angewandte Chemie (International Edition in English), 53(40), 10728-10732 (2014)
SIRT5 facilitates cancer cell growth and drug resistance in non-small cell lung cancer.
Lu W
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 35(11), 10699-10705 (2014)

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