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Documentos Principais

SRP3228

Sigma-Aldrich

PDGF-AA from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinônimo(s):

Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)

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About This Item

Código UNSPSC:
12352202
eCl@ss:
32190102
NACRES:
NA.32

fonte biológica

mouse

recombinante

expressed in E. coli

Ensaio

≥98% (HPLC)
≥98% (SDS-PAGE)

Formulário

lyophilized

potência

8-10 ng/mL ED50

peso molecular

28.7 kDa

embalagem

pkg of 10 μg

técnica(s)

cell culture | mammalian: suitable

Impurezas

<0.1 EU/μg endotoxin, tested

cor

white to off-white

nº de adesão UniProt

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

mouse ... PDGFA(18590)

Descrição geral

The PDGFs are stored in platelet α-granules and are released upon platelet activation. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids). Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids).

Aplicação

PDGF-AA from mouse has been used as a supplement in the media used for differentiation of neurospheres.

Ações bioquímicas/fisiológicas

The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-AA is crucial for optimal wound healing.

Sequência

MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT

forma física

Lyophilized with no additives.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1


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Visite a Biblioteca de Documentos

Interleukin-1 mitogenic activity for fibroblasts and smooth muscle cells is due to PDGF-AA.
Raines EW
Science, 243, 393-396 (1989)
Isolation of neural stem cells from the postnatal cerebellum.
Lee A
Nature Neuroscience, 8, 723-729 (2005)
Detection of platelet-derived growth factor (PDGF)-AA in actively healing human wounds treated with recombinant PDGF-BB and absence of PDGF in chronic nonhealing wounds.
Pierce GF
The Journal of Clinical Investigation, 96, 1336-1350 (1995)
Native and oxidized LDL enhances production of PDGF AA and the surface expression of PDGF receptors in cultured human smooth muscle cells.
Stiko-Rahm A
Arteriosclerosis and Thrombosis, 12, 1099-1109 (1992)
Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities.
Leveen P
Genes & Development, 8, 1875-1887 (1994)

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