SRP3119
NANOG human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
Produtos recomendados
fonte biológica
human
recombinante
expressed in E. coli
Ensaio
≥98% (HPLC)
≥98% (SDS-PAGE)
forma
lyophilized
peso molecular
34.5 kDa
embalagem
pkg of 20 μg
técnica(s)
cell culture | mammalian: suitable
Impurezas
<0.1 EU/μg endotoxin, tested
cor
white to off-white
nº de adesão UniProt
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Informações sobre genes
human ... NANOG(79923)
Descrição geral
NANOG gene is localized to human chromosome 12p13.31 and has multiple pseudogenes. This gene is composed of four exons, three introns, and a 915bp open reading frame (ORF). The encoded protein was first discovered in ESCs (embryonic stem cells) and is a divergent homeobox domain protein. NANOG protein is composed of 305 amino acids and a central homeodomain (HD). This HD is composed of an unstructured N-terminal arm, a bundle of three α-helices (H1–H3) interlinked by loops.
Recombinant human Nanog is a 34.7kDa protein, which is synthesized as a 304 amino acid polypeptide lacking a signal sequence for secretion.
Recombinant human Nanog is a 34.7kDa protein, which is synthesized as a 304 amino acid polypeptide lacking a signal sequence for secretion.
Ações bioquímicas/fisiológicas
NANOG is one of the central genes regulating self-renewal and pluripotency capacities of embryonic stem cells (ESC). Constitutive expression of this gene prevents the differentiation of ESCs, and along with SOX2 (SRY-box 4) and OCT-4 (octamer-binding transcription factor 4), it regulates pluripotency-related gene expression and maintains the pluripotency of ESCs. During ESC differentiation both these genes are down-regulated, and gene profiling shows that the expression of NANOG is uniformly high in ESC lines. There is significant similarity between ESC and carcinoma in situ testis (CIS), including the high expression of NANOG. It is novel marker for testicular CIS and germ cell tumors. NANOG shows abnormal expression in a variety of human cancers, such as carcinomas of the brain, oral cavity, head and neck, breast, lung, liver, pancreas, kidney, gastric, cervix, ovary, prostate, and colon. This expression is also related to treatment resistance and poor survival of cancer patients.
Sequência
SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV
forma física
Lyophilized with 10 mM Acetic Acid.
Reconstituição
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Skin Irrit. 2
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours.
Hoei-Hansen CE et al
Histopathology, 47(1), 48-56 (2005)
Shuai Gong et al.
Molecular carcinogenesis, 54(9), 679-687 (2015-05-28)
As one of the key pluripotency transcription factors, NANOG plays a critical role in maintaining the self-renewal and pluripotency in normal embryonic stem cells. Recent data indicate that NANOG is expressed in a variety of cancers and its expression correlates
Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells.
Hayashi Y
Proceedings of the National Academy of Sciences of the USA, 112(15), 4666-4671 (2015)
Adam H Hart et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 230(1), 187-198 (2004-04-27)
The murine Nanog gene, a member of the homeobox family of DNA binding transcription factors, has been shown recently to maintain pluripotency of embryonic stem cells. We have used a sequence homology and expression screen to identify and clone the
Kaoru Mitsui et al.
Cell, 113(5), 631-642 (2003-06-06)
Embryonic stem (ES) cells derived from the inner cell mass (ICM) of blastocysts grow infinitely while maintaining pluripotency. Leukemia inhibitory factor (LIF) can maintain self-renewal of mouse ES cells through activation of Stat3. However, LIF/Stat3 is dispensable for maintenance of
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