SRP0257

PDE1A Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Sinônimo(s): Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1A, HDAM1, HSPDE1A, Phosphodiesterase 1A
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in baculovirus infected insect cells
• Ensaio: ≥70% (SDS-PAGE)
• Formulário: aqueous solution
• atividade específica: ≥1237 units/μg
• peso molecular: 88.3 kDa
• embalagem: pkg of 10 μg
• concentração: >0.02 mg/mL
• nº de adesão NCBI: NM_005019
• nº de adesão UniProt: P54750
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... PDE1A(5136)

Descrição

Descrição geral

Human PDE1A (GenBank Accession No. NM_005019), full length with N-terminal GST tag, MW = 88.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system

Aplicação

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definição da unidade

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Nota de preparo

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.