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Documentos Principais

SRP0246

Sigma-Aldrich

LKB1/MO25/STRAD Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Sinônimo(s):

STK11, Serine/threonine kinase 11

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.32

fonte biológica

human

recombinante

expressed in baculovirus infected insect cells

Ensaio

≥70% (SDS-PAGE)

Formulário

aqueous solution

peso molecular

49 kDa (LKB1)
 66 kDa (MO25)
 74 kDa (STRAD)

embalagem

pkg of 20 μg

condição de armazenamento

avoid repeated freeze/thaw cycles

concentração

>0.02 mg/mL

nº de adesão NCBI

NM_000455 (STK11)
NM_001003787 (STRAD)
NM_016289 (MO25)

nº de adesão UniProt

Q15831

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... STK11(6794)

Categorias relacionadas

Descrição geral

The product is a complex of human Liver kinase B1 (LKB1), also known as serine/threonine kinase 11 (STK11) (GenBank Accession No. NM_000455; amino acids 2-433, with N-terminal His-tag), mouse protein- 25 (MO25) or calcium binding protein 39 (CAB39) (GenBank Accession No. NM_016289; amino acids 2-341, with N-terminal GST-tag) and STE20-related kinase adaptor alpha (STRAD) (GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag).

Aplicação

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Ações bioquímicas/fisiológicas

Liver kinase B1 (LKB1), also known as serine/threonine kinase 11 (STK11), is a serine/threonine kinase that regulates cell polarity and functions as a tumor suppressor protein. LKB1 regulates the activity of adenosine monophosphate-activated protein kinase (AMPK), promoting the suppression of growth and proliferation under energetically unfavorable conditions. Mouse protein-25 (MO25) or calcium binding protein 39 (CAB39) is a scaffolding protein which modulates the activities of STE20 family protein kinases. The MO25a isoform is part of the LKB1 tumor suppressor complex. STE20-related kinase adaptor α (STRAD) is a pseudokinase. It forms a heterotrimeric complex with LKB1 and MO25. The LKB1/MO25/STRAD complex modulates the metabolism, proliferation and cellular polarity of human intestinal epithelial cells.

forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota de preparo

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
4006940724
40069100524
4006900524

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Visite a Biblioteca de Documentos

Structural insights into the activation of MST3 by MO25.
Mehellou Y
Biochemical and Biophysical Research Communications, 431(3), 604-609 (2013)
P A Marignani et al.
Cancer biology & therapy, 6(10), 1627-1631 (2007-10-09)
STRADalpha is a pseudokinase that forms a heterotrimeric complex with the scaffolding protein MO25 and the tumor suppressor serine threonine protein kinase LKB1. Mutations in the LKB1 gene are responsible for the Peutz-Jeghers Syndrome (PJS) characterized by a predisposition to
Yue Zong et al.
Cell research, 29(6), 460-473 (2019-04-06)
AMPK, a master regulator of metabolic homeostasis, is activated by both AMP-dependent and AMP-independent mechanisms. The conditions under which these different mechanisms operate, and their biological implications are unclear. Here, we show that, depending on the degree of elevation of
Naomi X Y Ling et al.
Nature metabolism, 2(1), 41-49 (2020-01-30)
Central to cellular metabolism and cell proliferation are highly conserved signalling pathways controlled by mammalian target of rapamycin (mTOR) and AMP-activated protein kinase (AMPK)1,2, dysregulation of which are implicated in pathogenesis of major human diseases such as cancer and type

Artigos

Oncogenes and Tumor Suppressors Reprogram Metabolism

We present an article about how proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication.

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