SRP0142

PRMT3 human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

• Sinônimo(s): Arginine methyltransferase 3, HMT1 hnRNP methyltransferase-like 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like 3
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in E. coli
• Ensaio: ≥60% (SDS-PAGE)
• Formulário: aqueous solution
• peso molecular: 86 kDa
• embalagem: pkg of 50 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• concentração: >0.02 mg/mL
• nº de adesão NCBI: NM_005788
• nº de adesão UniProt: O60678
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... PRMT3(10196)

Descrição

Descrição geral

Human PRMT3, GenBank Accession No. NM_005788, amino acids 2-end, with N-terminal GST tag, MW = 86 kDa, expressed in an E. coli expression system.

Aplicação

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definição da unidade

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Nota de preparo

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.