SRE0116

Salt Active Nuclease (SAN)

recombinant, expressed in Pichia pastoris, Triton-free

• Sinônimo(s): endonuclease, recombinant nuclease, salt active endonuclease, salt tolerant nuclease
• Código UNSPSC: 12352204
• NACRES: NA.77

Propriedades

• recombinante: expressed in Pichia pastoris
• Nível de qualidade: 200
• composição: Triton-free
• embalagem: pkg of 25 kU
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

This Salt Active Nuclease (SAN) has been developed to offer the optimal solution for removal of nucleic acids at the near-physiological conditions used in many bioprocessing and biomanufacturing workflows. This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM. It is recombinantly expressed in Pichia pastoris.

Aplicação

• Can be used directly in cell medium or harvested supernatant without buffer adjustments
• Ideal for DNA removal directly in mammalian cell media

Características e benefícios

• Active at physiological conditions
• Endotoxin tested Animal origin free without buffer adjustments.

Definição da unidade

One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCl pH 7.6 (@25 °C), 2.5 mM MgCl₂, 150 mM NaCl, and 50 μg/ml calf thymus DNA. 25 mM Tris-HCl pH 7.6 (@25 °C), 2.5 mM MgCl₂, 150 mM NaCl, and 50 μg/ml calf thymus DNA

Reconstituição

In solution

Outras notas

• Temperature: 25 – 50 °C
• Salt concentration (NaCl): 50 – 400 mM, optimal: 125 – 250 mM
• Mg²⁺: > 0.5 mM is required for activity, optimal: 4 - 15 mM
• pH: 6.5 – 9.5, optimal: 7.2 - 8.7
• Not recommended to use in presence of EDTA or urea.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable