SAE0200
MycoPolyzyme
for use with yeast and fungal lysis, free of DNA contaminants, suitable for Microbiome research
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About This Item
Código UNSPSC:
12352204
NACRES:
NA.77
Produtos recomendados
Nível de qualidade
Formulário
lyophilized powder
Características
DNA free
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Descrição geral
Lytic Enzyme Mixture is a mix of two enzymes: lyticase and chitinase, which have been proven useful in fungal and yeast lysis for microbiome studies. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source. The mixture of the two enzymes has also been tested to ensure absence of microbial DNA.
Aplicação
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. Lytic Enzyme Mixture undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Componentes
The enzymes in Lytic Enzyme Mixture are:
The enzymes are tested for absence of contaminating microbial DNA using 16S and 18S PCR amplification.
- Lyticase
- Chitinase
The enzymes are tested for absence of contaminating microbial DNA using 16S and 18S PCR amplification.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
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Lot/Batch Number
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