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SAE0075

Sigma-Aldrich

Angiotensin Converting Enzyme, ACE, human

recombinant, ≥10 U/mg, expressed in HEK 293 cells

Sinônimo(s):

ACE, ACE1, CD143, DCP, DCP1, Dipeptidyl carboxypeptidase I, Kininase II, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase

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About This Item

Número da licença da enzima:
3.4.15.1 (BRENDA, IUBMB)
Código UNSPSC:
12352200
NACRES:
NA.77

recombinante

expressed in HEK 293 cells

Nível de qualidade

200

Ensaio

≥95% (SDS-PAGE)

Formulário

lyophilized powder

atividade específica

≥10 U/mg

peso molecular

calculated mol wt 138 kDa (The protein migrates as a 180 kDa protein on SDS-PAGE due to glycosylation)

nº de adesão UniProt

P12821

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

Recombinant human Angiotensin Converting Enzyme (ACE) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 138 kDa (amino acids 30-1232). The DTT-reduced protein migrates as a 180 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
The ACE gene codes for angiotensin-converting enzyme, which is one of the major enzymes in the RAS (renin angiotensin system). The ACE gene is localized on human chromosome 17q23.

Especificidade

The specific activity of recombinant human ACE is measured by its ability to cleave hippuryl-HIS-LEU

Ações bioquímicas/fisiológicas

Angiotensin I converting enzyme (ACE) catalyzes the formation of angiotensin II from angiotensin I. It is a membrane-bound enzyme, which is expressed in atherosclerotic lesions. It plays an important role in the development of various diseases like systemic lupus erythematosus, hypertension, chronic kidney disease and diabetic nephropathy.

Definição da unidade

One unit is defined as the amount of enzyme required to produce 1.0 micromole of hippuric acid from hippuryl-HIS-LEU per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 oC.

forma física

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
097M4873V
097M4874V
097M4875V

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Angiotensin-Converting Enzyme in Smooth Muscle Cells Promotes Atherosclerosis
Chen X, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology (2016)
Genetic Polymorphism of Angiotensin-Converting Enzyme and Chronic Obstructive Pulmonary Disease Risk: An Updated Meta-Analysis.
Kang SW, et al.
BioMed Research International (2016)
ACE Gene I/D Polymorphism and Obesity in 1,574 Patients with Type 2 Diabetes Mellitus.
Pan YH, et al.
Disease Markers (2016)
Local corticosterone production and angiotensin-I?converting enzyme shedding in a mouse model of intestinal inflammation
Salmenkari H, et al.
World Journal of Gastroenterology, 21(35), 10072-10072 (2015)
Yueru Li et al.
The American journal of pathology, 190(9), 1971-1981 (2020-06-27)
Leakage of retinal blood vessels, which is an essential element of diabetic retinopathy, is driven by chronic elevation of vascular endothelial growth factor (VEGF). VEGF quickly relaxes the endothelial cell barrier by triggering signaling events that post-translationally modify pre-existing components

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