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Key Documents

SAB4700663

Sigma-Aldrich

Monoclonal Anti-MHC Class II-FITC antibody produced in rat

clone M5/114, purified immunoglobulin, buffered aqueous solution

Sinônimo(s):

Rat Monoclonal Anti-MHC Class II-Fluorescein isothiocyanate

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rat

conjugado

FITC conjugate

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

M5/114, monoclonal

forma

buffered aqueous solution

reatividade de espécies

mouse

concentração

0.5 mg/mL

técnica(s)

flow cytometry: suitable

Isotipo

IgG2b

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Descrição geral

Major histocompatibility complex II (MHCII) is specifically localized on antigen presenting cells, such as macrophages, dendritic cells and B cells. MHC class II are classified in to two types classical and non- classical MHC class II molecules. In humans, there are three classical class II molecules HLA-DP, -DQ and –DR and two non-classical molecules, namely HLA-DM and –DO.
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Imunogênio

Activated C57BL/6 mouse spleen cells

Aplicação

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 4 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Ações bioquímicas/fisiológicas

Major histocompatibility complex II (MHCII) plays a vital role in initiating immune response by presenting peptides derived from extracellular pathogens to T cells bearing the CD4 marker. Mutations in the gene increase the risk of susceptibility to autoimmune diseases such as diabetes mellitus, rheumatoid arthritis and pemphigus vulgaris.

Características e benefícios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

forma física

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Li Dong et al.
Journal of inflammation research, 14, 2471-2482 (2021-06-19)
Parkinson's disease is a common neurodegenerative disease in the elderly. The incidence of various cancers in Parkinson's disease patients is significantly lower than in healthy people. Parkinson's disease patients are individuals with a high tendency for inflammation, whose peripheral immune
REGULATION OF MHC CLASS II GENES: Lessons from a Disease
Mach B, et al.
Annual Review of Immunology, 1996, 301-331 (1996)
Genetic Control of MHC Class II Expression
Ting JP and Trowsdale J
Cell, 109, S21-S33 (2002)
A molecular basis for MHC class II--associated autoimmunity
Todd JA, et al.
Science, 240, 1003-1009 (1988)
Yoshihiro Kuwano et al.
International immunology, 19(8), 977-992 (2007-09-07)
CD83 is a member of the Ig superfamily expressed primarily by mature dendritic cells (DCs). In mice, CD83 expression by thymic stromal cells regulates CD4(+) T cell development, with CD83(-/-) mice demonstrating dramatic reductions in both thymus and peripheral CD4(+)

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