SAB4200767

Anti-Cathepsin D antibody, Mouse monoclonal

clone CTD-19, purified from hybridoma cell culture

• Sinônimo(s): Anti-CTSD, cleaved into the following 2 chains: Cathepsin D light chain and Cathepsin D heavy chain
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 300
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: CTD-19, monoclonal
• forma: buffered aqueous solution
• reatividade de espécies: human, rabbit
• concentração: ~1.0 mg/mL
• técnica(s): immunoblotting: 2-4 μg/mL using human breast cancer MCF7 cell line; immunofluorescence: 5-10 μg/mL using HeLa cells; immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human liver sections
• Isotipo: IgG2a
• nº de adesão UniProt: P07339
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CTSD(1509)

Descrição

Descrição geral

Anti-Cathepsin D antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the CTD-19 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with cathepsin D purified from human liver. Cathepsin D (CatD or CTSD) is synthesized in the rough endoplasmic reticulum as pre-proprotein. After removal of signal peptide, the pro-CatD is targeted to endosomes to form an active, ~48 kDa, single-chain intermediate then to the lysosomes to form the fully active mature protease, composed of a ~30 kDa heavy chain and a ~14 kDa light chain.

Imunogênio

Cathepsin D Purified from human liver

Aplicação

Anti-Cathepsin D antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunohistochemistry
• immunofluorescence

Ações bioquímicas/fisiológicas

CatD plays numerous physiological functions in the cells including metabolic degradation of intracellular proteins and the activation of enzymatic precursors. In the central nervous system, CatD is particularly important for the control of neuronal homeostasis, cell migration and interneuron communication. CatD-mediated proteolysis mediates the degradation of unfolded/oxidized protein aggregates in lysosome. The level of CatD synthesized by the cells is increased in response to mitogenic signals from estrogen, epidermal growth factor (EGF), fibroblast growth factor (FGF), and insulin like growth factor-I (IGF- I). The ability of tumor cells to invade the extracellular matrix has been attributed to cathepsins released by tumor cells or associated with its plasma membrane.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable