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SAB4200695

Sigma-Aldrich

Anti-VSV Glycoprotein antibody, Mouse monoclonal

clone P5D4, purified from hybridoma cell culture

Sinônimo(s):

VSV, VSV glycoprotein, Vesicular Stomatitis Virus glycoprotein

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

mouse

Nível de qualidade

200

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

P5D4, monoclonal

Formulário

buffered aqueous solution

reatividade de espécies

virus (Vesicular stomatitis virus (VSV))

embalagem

antibody small pack of 25 μL

concentração

~1 mg/mL

técnica(s)

immunoblotting: 0.5-1 μg/mL using whole extract of human HEK-293T cells over-expressing Vinculin with VSV-G tagged fusion protein.
immunocytochemistry: suitable
immunofluorescence: 5-10 μg/mL using COS7 cells over-expressing Vinculin with VSV-G tagged fusion protein.
immunoprecipitation (IP): suitable

Isotipo

IgG1

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Descrição geral

Anti-VSV Glycoprotein antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma P5D4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.

Aplicação

Anti-VSV Glycoprotein antibody, Mouse monoclonal has been used in immunoblotting, immunofluorescence, immunoprecipitation, immunocytochemistry, transmission electron microscopy (TEM) and studies applying microinjection of the antibody.

Ações bioquímicas/fisiológicas

Vesicular Stomatitis Virus Glycoprotein (VSV-G) constitutes an attractive model to study maturation and intracellular transport of membrane proteins. It mediates attachment of VSV to the cell surface and induces pH-dependent fusion between viral and target membranes. In addition, it possesses information for intracellular sorting in its cytoplasmic domain, including efficient export from the endoplasmic reticulum (ER), basolateral delivery and endocytosis. The unidirectional transport between golgi cisternae was demonstrated using golgi membranes containing VSV-G. Temperature sensitive mutants of VSV-G are used to study exit of folding intermediates from the endoplasmic reticulum.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
019M4896V
078M4885V
22180332
018M4841V
057M4842V

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C Hammond et al.
The Journal of cell biology, 126(1), 41-52 (1994-07-01)
Proteins synthesized in the ER are generally transported to the Golgi complex and beyond only when they have reached a fully folded and assembled conformation. To analyze how the selective retention of misfolded proteins works, we monitored the long-term fate
T E Kreis et al.
Cell, 46(6), 929-937 (1986-09-12)
Using ts045, a temperature sensitive strain of Vesicular stomatitis virus, we show that oligomerization of G protein is a prerequisite for its transport from RER to the Golgi apparatus and for its subsequent maturation. While wild-type G forms an oligomer
T Soldati et al.
Cell, 66(2), 277-289 (1991-07-26)
The isoprotein-specific intracompartmental sorting of the three essential myosin light chains (LCs), the skeletal muscle LC-1f and LC-3f and the nonmuscle LC-3nm, was investigated. Epitope tagging was used to monitor the intracellular localization to different cytoskeletal structures of the exogenously
p38: A novel protein that associates with the vesicular stomatitis virus glycoprotein
Sevier CS and Machamer CE
Biochemical and Biophysical Research Communications, 287(2), 574-582 (2001)
C S Sevier et al.
Molecular biology of the cell, 11(1), 13-22 (2000-01-19)
The vesicular stomatitis virus (VSV) G protein is a model transmembrane glycoprotein that has been extensively used to study the exocytotic pathway. A signal in the cytoplasmic tail of VSV G (DxE or Asp-x-Glu, where x is any amino acid)
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