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Key Documents

SAB4200503

Sigma-Aldrich

Anti-PSPC1 antibody, Mouse monoclonal

enhanced validation

clone 1L4, purified from hybridoma cell culture

Sinônimo(s):

Monoclonal Anti-PSP1, Monoclonal Anti-PSPC1 antibody produced in mouse, Monoclonal Anti-paraspeckle component 1

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

primary antibodies

clone

1L4, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~60 kDa

reatividade de espécies

human, rat, mouse

validação aprimorada

independent
Learn more about Antibody Enhanced Validation

concentração

~1.0 mg/mL

técnica(s)

indirect immunofluorescence: 5-10 μg/mL using Human HeLa cells.
western blot: 0.5-1 μg/mL using Whole extracts of mouse Hepa1-6 cells.

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PSPC1(55269)
mouse ... Pspc1(66645)
rat ... Pspc1(305910)

Descrição geral

Monoclonal Anti-PSPC1 (mouse IgG1 isotype) is derived from the hybridoma 1L4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of mouse PSPC1. DBHS (Drosophila behavior, human splicing) protein family, consisting also of NONO (nonPOU-domain-containing, octamer binding protein) and SFPQ (splicing factor, praline- and glutamine-rich). NONO, paraspeckle component 1 (PSPC1) and SFPQ are the core protein components of paraspeckles. These multi-functional nuclear proteins, under normal conditions, cycle between the nucleoplasm, paraspeckles and the nucleolus.
Paraspeckle component 1 (PSPC1) is encoded by the gene mapped to human chromosome 13q12.11. The encoded protein is a member of DBHS (Drosophila Behavior Human Splicing) family of proteins.

Imunogênio

Synthetic peptide corresponding to a sequence at the C-terminal region of mouse PSPC1, conjugated to KLH. 1 The corresponding sequence is identical in rat and human PSPC1.

Aplicação

Monoclonal Anti-PSPC1 antibody produced in mouse has been used in immunohistochemistry, immunoblotting and immunofluorescence.

Ações bioquímicas/fisiológicas

Drosophila behavior, human splicing (DBHS) proteins bind both double- and single-stranded DNA and RNA, and are involved in various aspects of RNA and DNA metabolism, such as transcription, pre-mRNA 3′ processing, transcription termination and mRNA splicing. DBHS proteins regulate circadian rhythm and carcinogenesis. PSPC1 activates androgen receptor mediated transcription. PSPC1 forms a heterodimer with NONO that localizes to paraspeckles in a RNA polymerase II-dependent manner.
Since paraspeckle component 1 (PSPC1) has lower background nucleoplasmic signal in various cell types compared to other members of DBHS (Drosophila Behavior Human Splicing) family of proteins, it is frequently used as a marker for paraspeckles. The encoded protein plays a vital role in regulation of transcription. Deletion of the gene is associated with the hindered psychomotor development, muscle hypotonia, unilateral microphthalmia with ptosis, congenital eye malformation and facial dysmorphic features.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Visite a Biblioteca de Documentos

Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation
Passon DM, et al.
Proceedings of the National Academy of Sciences of the USA, 109(13), 4846-4850 (2012)
First-trimester euploid miscarriages analysed by array-CGH.
Viaggi CD
Journal of applied genetics, 54, 353-359 (2013)
Neat1 in hematopoietic stem cells
Fallik N, et al.
Oncotarget, 8(65), 109575-109575 (2017)
Noam Fallik et al.
Oncotarget, 8(65), 109575-109586 (2018-01-10)
Hematopoietic Stem Cells (HSCs) generate blood and immune cells through a hierarchical process of differentiation. Genes that regulate this process are of great interest for understanding normal and also malignant hematopoiesis. Surprisingly, however, very little is known about long-non-coding RNAs
Paraspeckles
Fox A H
Cold Spring Harbor Perspectives in Biology, 2 (2010)

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