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SAB2702215

Sigma-Aldrich

Monoclonal Anti-Strep Tag antibody produced in mouse

clone GT517, affinity isolated antibody

Sinônimo(s):

Mouse Anti-Strep Tag, Strep Tag Detection Antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43
conjugado:
unconjugated
application:
IF
WB
clone:
GT517, monoclonal
técnica(s):
indirect immunofluorescence: suitable
western blot: 1000-10000
citations:
8

fonte biológica

mouse

Nível de qualidade

100

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

GT517, monoclonal

Formulário

buffered aqueous solution

concentração

1mg/mL

técnica(s)

indirect immunofluorescence: suitable
western blot: 1000-10000

Isotipo

IgG1

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Descrição geral

The strep-tag/strep-tagII (improved version) is made up of eight amino acid sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys).

Imunogênio

The immunogen used to generate this antibody corresponds to Strep Tag

Aplicação

Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Ações bioquímicas/fisiológicas

The strep-tag/strep-tagII (improved version) has the ability to bind to any protein or peptide of interest.

Características e benefícios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Outras notas

Purification: Affinity purified by Protein G

forma física

Phosphate-buffered saline, no preservative added.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
41246

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The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins.
Schmidt TG and Skerra A
Nature Protocols, 2(6), 1528-1528 (2007)
Zhaofeng Yan et al.
Nature communications, 11(1), 241-241 (2020-01-15)
A major component of bacterial biofilms is curli amyloid fibrils secreted by the curli biogenesis system. Understanding the curli biogenesis mechanism is critical for developing therapeutic agents for biofilm-related infections. Here we report a systematic study of the curli biogenesis
The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture.
Schmidt T and Skerra A
Methods in Molecular Biology, 1286, 83-95 (2015)
Timothy A Bates et al.
Cell reports, 34(7), 108737-108737 (2021-02-06)
In the ongoing coronavirus disease 2019 (COVID-19) pandemic, there remain unanswered questions regarding the nature and significance of the humoral immune response toward other coronavirus infections. Here, we investigate the cross-reactivity of antibodies raised against the first severe acute respiratory
Yongqiang Lai et al.
Scientific reports, 9(1), 14257-14257 (2019-10-05)
Replication protein A (RPA) binds to and stabilizes single-stranded DNA and is essential for the genome stability. We reported that an E3 ubiquitin ligase, HERC2, suppresses G-quadruplex (G4) DNA by regulating RPA-helicase complexes. However, the precise mechanism of HERC2 on

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