S8070

Anti-SUMO-1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinônimo(s): Anti-GMP1, Anti-PIC1, Anti-SMT3C, Anti-SMT3H3, Anti-Sentrin-1, Anti-Small Ubiquitin-related Modifiier-1, Anti-UBL1
• Número MDL: MFCD02096449
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• peso molecular: antigen 14 kDa (unconjugated SUMO-1); antigen 41 kDa (SUMO-1-GST)
• reatividade de espécies: human
• técnica(s): immunoprecipitation (IP): 10-20 μg using a nuclear extract of HeLa cells; indirect immunofluorescence: 4-8 μg/mL using human HeLa cells; microarray: suitable; western blot: 0.5-1 μg/mL using nuclear extract of HeLa cells
• nº de adesão UniProt: P63165
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: cow ... SUMO1(614967) ; human ... SUMO1(7341) ; mouse ... Sumo1(22218) ; rat ... Sumo1(301442)

Descrição

Descrição geral

The SUMO-1 (small ubiquitin-like modifier 1) gene is mapped to human chromosome 2q33.1 and is highly conserved. The protein structure includes an α-helix core surrounded by four-stranded β-sheets.

Imunogênio

synthetic peptide corresponding to amino acid 1-16 located at the N-terminus of human SUMO-1, conjugated to KLH. The sequence is identical in many species including mouse, rat, dog, bovine, and highly conserved (single amino acid substitution) in chicken and Xenopus SUMO-1. It is not found in human SUMO-2 and SUMO-3.

Aplicação

Anti-SUMO-1 antibody produced in rabbit has been used in immunoblotting assay.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Ações bioquímicas/fisiológicas

SUMO (small ubiquitin-like modifier) proteins are associated with a number of cellular processes. SUMOylation mediated by SUMO1 is involved in nuclear transport mechanism, apoptosis, regulation of cyclin-dependent kinase 6 and protein stability. SUMO1 regulates tumor suppressor p53 protein, a transcription factor that is termed as the “guardian of the genome” plays a key role in the cell cycle events. Unlike ubiquitination which directs proteins for degradation, SUMOylation aims to alter the stability, localization and the function of the target protein.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)