S7320

Anti-Spred-2 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinônimo(s): Anti-Sprouty Protein with EVH-1 Domain 2
• Número MDL: MFCD07784893
• Código UNSPSC: 12352203
• NACRES: NA.44

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• peso molecular: ~48 kDa
• reatividade de espécies: human, mouse, rat
• concentração: ~1 mg/mL
• técnica(s): indirect immunofluorescence: 5-10 μg/mL using PC-12 cell lines; microarray: suitable; western blot: 1-2 μg/mL using cell extracts of HepG2; western blot: 2-4 μg/mL using mouse liver extracts
• nº de adesão UniProt: Q7Z698
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SPRED2(200734) ; mouse ... Spred2(114716) ; rat ... Spred2(305539)

Descrição

Descrição geral

Anti-Spred-2 is developed in rabbit using as immunogen a synthetic peptide corresponding to amino acids of mouse Spred-2, conjugated to KLH via an N terminal added cysteine residue. Spred-2, also known as sprouty protein with EVH-1 domain 2 related sequence, was isolated by similarity to Spred-1, which in turn was isolated from an osteoclast complementary DNA library by a two-hybrid system using c-Kit and c-Fms tyrosine kinase domains as bait. These proteins contain a carboxy-terminal cysteine-rich domain related to sprouty (the SPR domain), and an amino-terminal Ena/vasodilator stimulated phosphoprotein (VASP) homology (EVH-1) domain. This gene is mapped to human chromosome 2p14.

Spred proteins mainly function as inhibitors of the ERK and MAPK signaling cascades. Phosphorylation and Cbl-dependent ubiquitination regulate Spred-2 expression levels. Spred2 negatively modulates the hypothalamic-pituitary-adrenal axis . Anti-Spred-2 antibody is specific for human, rat, and mouse Spred-2 (approx. 48 kDa). Staining of the Spred-2 band in immunoblotting is specifically inhibited by the immunizing peptide.

Imunogênio

synthetic peptide corresponding to amino acids 280-297 of mouse Spred 2. The sequence is conserved in rat and differs from human by three amino acids.

Aplicação

Anti-Spred-2 antibody produced in rabbit has been used in western blotting and indirect immunofluorescence.

Ações bioquímicas/fisiológicas

Spred inhibits growth factor mediated activation of MAP kinase. Spred inhibits the activation of MAP kinase probably by mechanisms such as suppression of phosphorylation and activation of Raf. Spred-2 functions as a negative regulator of AGM (aorta-gonad-mesonephros) hematopoiesis by inhibiting hematopoietic cytokine signaling. Spred proteins are also regulators of Rho-mediated cell motility.

forma física

Solution in 0.01 M phoshate buffered saline, pH 7.4, 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)