S6436
Anti-phospho-S6-Kinase (pThr421/pSer424) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Sinônimo(s):
Anti-phospho-p70S6K (pThr421/pSer424)
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About This Item
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
Formulário
buffered aqueous glycerol solution
reatividade de espécies
mouse, rat, human
técnica(s)
immunoprecipitation (IP): 1:250
western blot (chemiluminescent): 1:1,000
nº de adesão UniProt
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Informações sobre genes
human ... RPS6KB1(6198)
mouse ... Rps6kb1(72508)
rat ... Rps6kb1(83840)
Categorias relacionadas
Descrição geral
S6 kinase (p70s6k) is activated by an upstream kinase, mTOR in response to nutrients and growth factors. S6 kinase (p70s6k) is a critical regulatory component and mTOR along with the eukaryotic initiation factor 4E (eIF4E)–binding protein 1 (4E-BP1) and S6 kinase (p70s6k) leads to increased ribosomal biogenesis and hence translation. As a critical component and has the ability to modify the cellular responses to availability of nutrients, and growth factors like insulin and IGF-1 when mTOR is chronically active. In presence of excess and chronic insulin, or high fat diets, S6 kinase (p70s6k) regulates by a negative feedback loop and constitutively shuts down the responsiveness of cells to insulin. Thus, mTOR through the S6 kinase (p70s6k) plays a pivotal role in the development of insulin resistance and type 2 diabetes. mTOR signaling via p70S6 kinase also drives cell growth and proliferation, cancer and aging.
Anti-phospho-S6-kinase (p70S6K) (phosphothreonine 421/phosphoserine424) detects p70 S6 kinase and p85 S6 kinase only when activated by phosphorylation at Thr421/Ser424 and does not cross-react with other phosphoylated protein kinases.
Anti-phospho-S6-kinase (p70S6K) (phosphothreonine 421/phosphoserine424) detects p70 S6 kinase and p85 S6 kinase only when activated by phosphorylation at Thr421/Ser424 and does not cross-react with other phosphoylated protein kinases.
Especificidade
Does not react with non-phosphorylated S6 kinase (p70S6K) or other phosphorylated protein kinases.
Imunogênio
synthetic double phospho-Thr421/Ser424 peptide corresponding to residues around Thr421/Ser424 of human p70S6K.
Aplicação
Anti-phospho-S6-kinase (p70S6K) may be used for immunoblotting at a working dilution of 1:1000 in serum-treated NIH-3T3 cells. For immunoprecipitation, the recommended dilultion is 1:250.
forma física
Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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K Hara et al.
The Journal of biological chemistry, 273(23), 14484-14494 (1998-06-11)
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In all eukaryotes, the target of rapamycin (TOR) signalling pathway couples energy and nutrient abundance to the execution of cell growth and division, owing to the ability of TOR protein kinase to simultaneously sense energy, nutrients and stress and, in
Brendan D Manning
The Journal of cell biology, 167(3), 399-403 (2004-11-10)
Proper regulation of the phosphoinositide 3-kinase-Akt pathway is critical for the prevention of both insulin resistance and tumorigenesis. Many recent studies have characterized a negative feedback loop in which components of one downstream branch of this pathway, composed of the
Hongyu Zhou et al.
Current protein & peptide science, 11(6), 409-424 (2010-05-25)
The mammalian target of rapamycin (mTOR) has attracted substantial attention because of its involvement in a variety of diseases, such as cancer, cardiac hypertrophy, diabetes and obesity. Current knowledge indicates that mTOR functions as two distinct multiprotein complexes, mTORC1 and
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