S6197

Anti-STIM1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinônimo(s): Anti-GOK, Anti-Stromal interaction molecule 1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen 90 kDa
• reatividade de espécies: human, rat, mouse
• concentração: ~1 mg/mL
• técnica(s): immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells.; indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells.; western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells.
• nº de adesão UniProt: Q13586
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... STIM1(6786) ; mouse ... Stim1(20866) ; rat ... Stim1(117086)

Descrição

Descrição geral

Stromal interaction molecule 1 (STIM1) is a conserved single-pass transmembrane protein. STIM1 is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 localizes predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca²⁺ levels.

Imunogênio

synthetic peptide corresponding to amino acids 657-683 of human STIM1, conjugated to KLH via an N-terminal residue. The corresponding sequence is identical in rat, mouse, bovine, monkey, and dog.

Aplicação

Anti-STIM1 (C-terminal) antibody produced in rabbit has been used in:

• western blot
• immunoprecipitation
• immunofluorescence
• immunohistochemistry

Ações bioquímicas/fisiológicas

Stromal interaction molecule 1 (STIM1) is required for the activation of store-operated Ca²⁺ influx. STIM1 undergoes rapid oligomerization and redistributes into discrete spots (punctae) that move towards and accumulate in the cell periphery, possibly to activate Orai1 that is located in the plasma membrane. Overexpression of STIM1 and Orai1 together markedly increases the calcium release-activated channels (CRAC) current (I-CRAC). Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex plays a major role in the activation of store-operated calcium (SOC) channel in response to internal Ca²⁺ store depletion.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)