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Merck
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Documentos Principais

RAB0480

Sigma-Aldrich

Rat Tumor Necrosis Factor α ELISA Kit

for cell and tissue lysates

Sinônimo(s):

Cachectin, TNF-alpha, Tumor necrosis factor alpha

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

reatividade de espécies

rat

embalagem

kit of 96 wells (12 strips x 8 wells)

técnica(s)

ELISA: suitable
capture ELISA: suitable

entrada

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

método de detecção

colorimetric

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

rat ... Tnf(24835)

Descrição geral

The Rat TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF-α cell lysate and tissue lysate.

Imunogênio

Recombinant Rat TNF-α

Aplicação

For research use only. Not for use in diagnostic procedures.
Rat Tumor Necrosis Factor α ELISA Kit has been used to measure rat tumor necrosis-α (TNF-α) in cell lysate and tissue lysate.

Ações bioquímicas/fisiológicas

Tumor necrosis factor-α (TNF-α) affects various cellular processes such as blood-brain barrier, inflammatory, thrombogenic, and vascular changes following brain damage. TNF-α expression is upregulated in various central nervous system disorders, including Alzheimer′s disease, multiple sclerosis, Parkinson′s disease, meningococcal meningitis and HIV infection. Elevated expression of TNF-α in brain and blood in response to lipopolysaccharide might increase brain stem thrombosis and hemorrhage and stroke sensitivity/risk in hypertensive rats. Tumor necrosis factor-α activates polymorphonuclear leukocyte NADPH oxidase, thereby facilitates development of systemic oxidative stres (OS), inflammation and hypertension in rats.

Outras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABLYSIS12X Cell Lysis Buffer (Item J)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictogramas

Corrosion

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Met. Corr. 1

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials


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Tumor necrosis factor-alpha: a possible priming agent for the polymorphonuclear leukocyte-reduced nicotinamide-adenine dinucleotide phosphate oxidase in hypertension.
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