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RAB0448

Sigma-Aldrich

Cell-Based ELISA for detecting phospho-STAT4 (pTyr693) in cultured cell lines

96 assays

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About This Item

Código UNSPSC:
41116158

uso

96 assays

reatividade de espécies

mouse, human

técnica(s)

ELISA: suitable
capture ELISA: suitable

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... STAT4(6775)
mouse ... STAT4(20849)

Categorias relacionadas

Descrição geral

The Cell-Based Stat4 (pTyr693) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Stat4 (pTyr693) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human and mouse cell lines.

Imunogênio

Stat4 (pTyr693) synthetic phosphopeptide

Aplicação

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS
  • RABBLOCK5x Blocking Solution (Item F)SDS
  • RABFIX1Fixing Solution (Item D)SDS
  • RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDS
  • RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDS
  • RABPLATE1Uncoated MicroplateSDS
  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS
  • RABSTOP1Stop SolutionSDS
  • RABTMB1TMB Substrate Reagent (Item J)SDS
  • RABWASH120X Wash Buffer Concentrate ASDS
  • RABWASH220X Wash Buffer Concentrate BSDS
Ver tudo (10)

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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C M Bacon et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(16), 7307-7311 (1995-08-01)
Interleukin 12 (IL-12) is an important immunoregulatory cytokine whose receptor is a member of the hematopoietin receptor superfamily. We have recently demonstrated that stimulation of human T and natural killer cells with IL-12 induces tyrosine phosphorylation of the Janus family
K Yamamoto et al.
Molecular and cellular biology, 14(7), 4342-4349 (1994-07-01)
Interferon regulation of gene expression is dependent on the tyrosine phosphorylation and activation of the DNA-binding activity of two related proteins of 91 kDa (STAT1) and/or 113 kDa (STAT2). Recent studies have suggested that these proteins are substrates of Janus
Hua-Chen Chang et al.
The Journal of biological chemistry, 278(34), 32471-32477 (2003-06-14)
STAT4 (signal transducer and activator of transcription-4) mediates biological effects in response to interleukin-12 (IL-12). STAT4 has multiple domains that have distinct functions in signaling and gene activation. To characterize the role of the STAT4 N-terminal domain in mediating STAT4

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