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R8653

Sigma-Aldrich

Anti-ROCK-2 antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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About This Item

Número MDL:
MFCD07370479
Código UNSPSC:
12352203
NACRES:
NA.44

fonte biológica

rabbit

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

peso molecular

antigen 160 kDa

reatividade de espécies

human, mouse

concentração

~2 mg/mL

técnica(s)

immunoprecipitation (IP): 25-50 μg using Jurkat cell lysate
microarray: suitable
western blot: 2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line.

nº de adesão UniProt

O75116

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... ROCK2(9475)
mouse ... Rock2(19878)
rat ... Rock2(25537)

Descrição geral

ROCK2 is a serine/threonine kinase that regulates actin cytoskeletal framework. Absence of ROCK2 alleviates atherosclerosis, whereas its overexpression has been associated with hepatocellular carcinoma. Genetic mutations in ROCK2 has been linked to the risk of Behcet′s disease . Anti-ROCK-2 recognizes human and mouse ROCK-2 (160 kDa).

Imunogênio

The sequence is highly conserved in Xenopus ROCK-2.
synthetic peptide corresponding to amino acids 1371-1388 located at the C-terminus of human ROCK-2, conjugated to KLH. This sequence is identical in rat, mouse and bovine ROCK-2.

Aplicação

The antibody is suitable for use in immunoprecipitation (25-50 μg using Jurkat cell lysate), microarray and western blot (2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line). The antibody may also be used for immunofluorescence (3-6 μg/ml using NIH3T3 cell line).

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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ROCK2 Deficiency in Bone Marrow-Derived Cells Leads to Increased Cholesterol Efflux and Decreased Atherosclerosis.
Zhou, Q., et al.
Circulation, doi: 10-doi: 10 (2012)
Wei Chen et al.
Scientific reports, 8(1), 16636-16636 (2018-11-11)
Rho-associated coiled-coil kinase (ROCK)2 targeting down-regulates autoimmune responses in animal models and patients, however the underlying molecular mechanism is still an enigma. We report that ROCK2 binds phosphorylated-STAT3 and its kinase activity controls the formation of ROCK2/STAT3/JAK2 complex and optimal
Association between Rho-kinase (ROCK2) gene polymorphisms and Behcet's disease.
Oguz, E., et al.
Translational Research, doi: 10-doi: 10 (2012)
Tiande Liu et al.
Experimental cell research, 318(16), 1994-2003 (2012-06-19)
Rho-associated coiled-coil containing protein kinase 2 (Rock2) belongs to a family of serine/threonine kinases which are actived via interaction with Rho GTPases. Recently, overexpression of Rock2 has been demonstrated in human hepatocellular carcinoma (HCC), but the potential role of Rock2
He-Song Jiang et al.
International journal of molecular medicine, 36(3), 801-807 (2015-07-17)
The transformation of tunica albuginea-derived fibroblasts (TAFs) into myofibroblasts plays an important role in the pathological progress of Peyronie's disease (PD). However, no treatment which addresses this transformation is currently available. Estrogen has been shown to inhibit the progression of

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