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P7059

Sigma-Aldrich

Solução salina tamponada com fosfato

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

Sinônimo(s):

PBS

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About This Item

Número MDL:
Código UNSPSC:
12161700
eCl@ss:
32129211
NACRES:
NA.25

Nome do produto

Solução salina tamponada com fosfato, 10× PBS for Western blots and IP

grau

for molecular biology

Nível de qualidade

esterilidade

0.2 μm filtered

Formulário

liquid

técnica(s)

immunoprecipitation (IP): suitable
western blot: suitable

pH

7.2-7.6(1x solution)

temperatura de armazenamento

room temp

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Descrição geral

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Aplicação

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Componentes

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate

Nota de preparo

Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Sahar M Greish et al.
International journal of physiology, pathophysiology and pharmacology, 12(5), 134-146 (2020-11-24)
Hypertension can be induced by inhibiting nitric oxide synthesis with L-NAME, which also has a role in oxidative stress. Curcumin has strong antioxidant property. Our aim was to examine the possible preventive role of curcumin on renal dysfunction secondary to
Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney
Caroline R Archer et al.
Scientific reports, 8(1), 10160-10160 (2018-07-07)
Pharmaceutical agents despite their efficacy to treat disease can cause additional unwanted cardiovascular side effects. Cardiotoxicity is characterized by changes in either the function and/or structure of the myocardium. Over recent years, functional cardiotoxicity has received much attention, however morphological

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