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Key Documents

P0071

Sigma-Aldrich

Anti-PDCD4 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Anti-Neoplastic transformation inhibitor, Anti-Nuclear antigen H731, Anti-Programmed cell death 4, Anti-Protein 197/15a

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen ~51 kDa

reatividade de espécies

human, mouse (predicted), rat (predicted)

concentração

~1 mg/mL

técnica(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates
western blot: 2-4 μg/mL using K562 cell lysates

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PDCD4(272250)
mouse ... Pdcd4(18569)
rat ... SELP(6403)

Descrição geral

Programmed Cell Death 4 (PDCD4 ) is present in the nucleus. PDCD4 gene is mapped to human chromosome 10q25.2. PDCD4 protein consists of two basic domains at the N-terminus and C-terminus and two conserved α-helical MA-3 domains.

Especificidade

Anti-PDCD4 specifically recognizes human, mouse, and rat PDCD4.

Aplicação

Anti-PDCD4 antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Ações bioquímicas/fisiológicas

Programmed Cell Death 4 (PDCD4) is a neoplastic transformation inhibitor protein that functions as a tumor suppressor in ovarian carcinoma. The levels of PDCD4 are depleted in progressed carcinomas of the lung, breast, colon, and prostate. MA-3 domain of PDCD4 aids protein-protein interactions in eukaryotic translation initiation factor 4GI(eIF4GI) and eIF4GII with the ATP-dependent RNA helicase eIF4A. PDCD4 suppresses translation initiation by specifically inhibiting the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. In response to mitogens, PDCD4 is rapidly phosphorylated on Ser67 by the protein kinase ribosomal protein S6 kinase 1 (S6K1), and subsequently degraded via the ubiquitin ligase skp, cullin, f-box containing complex beta-transducin repeat-containing protein ((SCF)βTRCP ). It also shows that the protein is specifically phosphorylated on Ser76 and Ser457 by p90 ribosomal S6 kinase (RSK).

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Xialian Xu et al.
Kidney international, 82(11), 1167-1175 (2012-07-13)
Delayed ischemic preconditioning effectively protects kidneys from ischemia-reperfusion injury but the mechanism underlying renal protection remains poorly understood. Here we examined the in vivo role of microRNA miR-21 in the renal protection conferred by delayed ischemic preconditioning in mice. A
Brigitte Lankat-Buttgereit et al.
Biology of the cell, 101(6), 309-317 (2009-04-10)
Pdcd4 (programmed cell death 4) has been known as a tumour suppressor gene and potential target for anticancer therapies for several years. Initially, Pdcd4 was identified as a gene that is up-regulated during apoptosis, but its precise role still remains
Sachiko Matsuhashi et al.
International journal of molecular sciences, 20(9) (2019-05-12)
PDCD4 is a novel tumor suppressor to show multi-functions inhibiting cell growth, tumor invasion, metastasis, and inducing apoptosis. PDCD4 protein binds to the translation initiation factor eIF4A, some transcription factors, and many other factors and modulates the function of the
Nina Stenzel et al.
Journal of cell science, 122(Pt 18), 3374-3384 (2009-08-27)
Multi-PDZ (PSD-95/Discs large/Zonula-occludens-1) domain proteins play a crucial role in the establishment and maintenance of cell polarization. The novel multi-PDZ domain protein FRMPD2 is a potential scaffolding protein consisting of an N-terminal KIND domain, a FERM domain and three PDZ
Britta Walter et al.
Histochemistry and cell biology, 133(1), 69-83 (2009-10-16)
Protein p0071 is a member of the p120-subfamily of armadillo proteins and is well known as a junctional plaque component involved in cell-cell adhesion, especially in adherens junctions. By systematic immunohistochemical analysis of mouse and human kidney tissues, p0071 was

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