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Key Documents

O9515

Sigma-Aldrich

Prolyl oligopeptidase

recombinant, expressed in E. coli

Sinônimo(s):

Prolyl endopeptidase

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.54

recombinante

expressed in E. coli

Nível de qualidade

Ensaio

≥90% (SDS-PAGE)

forma

solution

atividade específica

≥10 unit/μg protein

peso molecular

81.6 kDa

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Descrição geral

Prolyl oligopeptidase (PO) is mapped to human chromosome 6q22. It comprises of a N-terminal β-propeller domain and C-terminal α/β hydrolase catalytic domain. PO is highly expressed in the neuronal cytoplasm and its expression increases with age.
Prolyl oligopeptidase is a cytosolic serine peptidase which cleaves peptide bonds at the C′ terminal side of prolines. It is only capable of processing peptides containing no more than 30 amino acids due to the unique β-propeller region that regulates access to the active site.

Aplicação

Prolyl oligopeptidase has been used in a study to assess the mechanism of lithium ion action. It has also been used in a study to investigate its distribution in human tissue and body fluids.
Prolyl oligopeptidase has been used in the prolyl oligopeptidase inhibitory activity assay in lyophilized protein hydrolysate samples and Schistosoma mansoni samples.

Ações bioquímicas/fisiológicas

Prolyl oligopeptidase (PO) regulates physiological processes and is crucial for the generation of active hormone and peptide fragments from their precursors. It shows elevated levels in psychiatric disorders and Alzheimer′s patients. Altered levels of PO is observed in patients with bipolar disorder. High expression of PO may promote metastasis of malignant ovarian and colorectal tumors.

Definição da unidade

One unit will hydrolyze 1.0 picomole of Ala-Pro-aminomethylcoumarin per minute at pH 7.4 at 25 °C.

forma física

Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT and variable amounts of imidazole.

Nota de preparo

N-terminal GST-tagged 81.6 kDa full-length protein

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Prolyl oligopeptidase and bipolar disorder
Williams, Robin SB
Clinical neuroscience research, 4(3-4), 233-242 (2004)
L Polgár
Cellular and molecular life sciences : CMLS, 59(2), 349-362 (2002-03-28)
A group of serine peptidases, the prolyl oligopeptidase family, cannot hydrolyze peptides containing more than about 30 residues. This group is unrelated to the classical trypsin and subtilisin families, and includes dipeptidyl peptidase IV, acylaminoacyl peptidase and oligopeptidase B, in
Distribution of Prolyl Oligopeptidase in Human Peripheral Tissues and Body Fluids
Goossens, F., et al.
Clinical Chemistry and Laboratory Medicine, 34, 17-22 null
Prolyl oligopeptidase from the blood fluke Schistosoma mansoni: from functional analysis to anti-schistosomal inhibitors
Fajtova P, et al.
PLoS Neglected Tropical Diseases, 9(6), e0003827-e0003827 (2015)
Distribution of prolyl oligopeptidase in human peripheral tissues and in ovarian and colorectal tumors
Myohanen TT, et al.
The Journal of Histochemistry and Cytochemistry, 60(9), 706-715 (2012)

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