MTOX1010
HepaRG™ 5F Control Cells
human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
Sinônimo(s):
Human hepatocyte cells
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.24
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Nome do produto
HepaRG™ 5F Control Cells, 1 vial
fonte biológica
human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
Formulário
liquid
temperatura de armazenamento
−196°C
Descrição geral
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
This product consists of ZFN engineered HepaRG 5F clone control cells. They are intended for use as both control cells for HepaRG™ knockout cells as well as for a wide variety of liver cell based assays. Hepa RG cells display hepatocyte-like functions and can be a replacement to primary human hepatocytes in a number of drug metabolism, disposition and genotoxicity studies.
Aplicação
HepaRG™ 5F Control Cells has been used in a gene expression assay to study the effect of pregnane X receptor on the induction of human cytochrome P450 3A4 by the irreversible myeloperoxidase inactivator PF-06282999.
See technical bulletins for detailed protocols
Características e benefícios
Sigma′s HepaRG 5F Clone exhibits improved growth characteristics and enhanced functionality, expressing a large panel of drug metabolism enzymes; including cytochrome P450 enzymes CYP1A2, CYP2B6, CYP2C8/9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4, nuclear receptors AhR, PXR, CAR, LXR, and FXR, and phase II enzymes UGT, SULT, NAT and GST.
Qualidade
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation
Informações legais
These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company
Exoneração de responsabilidade
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Assessment of the genotoxic potential of indirect chemical mutagens in HepaRG cells by the comet and the cytokinesis-block micronucleus assays.
Le Hegarat, Ludovic, et al.
Mutagenesis, 25, 555-560 (2010)
The HepaRG cell line: a unique in vitro tool for understanding drug metabolism and toxicology in human.
Andersson, Tommy
Expert Opinion on Drug Metabolism & Toxicology, 8, 909-920 (2012)
Induction of human cytochrome P450 3A4 by the irreversible myeloperoxidase inactivator PF-06282999 is mediated by the pregnane X receptor.
Moscovitz, Jamie E., et al.
Xenobiotica, 1-9 (2017)
Daochuan Li et al.
Biochemical pharmacology, 98(1), 190-202 (2015-08-16)
The constitutive androstane receptor (CAR) modulates the transcription of numerous genes involving drug metabolism, energy homeostasis, and cell proliferation. Most functions of CAR however were defined from animal studies. Given the known species difference of CAR and the significant cross-talk
Wenwei Lin et al.
Molecular pharmacology, 97(3), 180-190 (2019-12-29)
The xenobiotic receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are activated by structurally diverse chemicals to regulate the expression of target genes, and they have overlapping regulation in terms of ligands and target genes. Receptor-selective agonists are
Artigos
Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic circulation.
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